African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Bacterial community composition of a saline-alkaline soil amended or not with wastewater sludge and contaminated with polycyclic aromatic hydrocarbons (PAHs)

  Fabián Fernández-Luqueño1*, Edgar Vázquez-Núñez2, Francisco J. Zavala-Díaz de la Serna3, Conrado Martínez-Suárez2, Gabriela Salomón-Hernández2, Cesar Valenzuela-Encinas2, Olivia Franco Hernández4, Juan Manuel Ceballos-Ramírez2 and Luc Dendooven2
  1Natural Resources and Energy Group, Cinvestav-Saltillo, Coahuila, C.P. 25900, México. 2Department of Biotechnology and Bioengineering, Cinvestav, México D.F, C.P. 07360, México. 3Facultad de Ciencias Químicas-UACH, Chihuahua, Chih. C.P. 31125, México. 4Instituto Politécnico Nacional. UPIBI, México D.F., C.P. 07340, México.
Email: [email protected]

  •  Accepted: 03 June 2013
  •  Published: 12 July 2013



The purpose of this study was to determinate the dynamics of the bacterial community composition, that is, diversity and abundance of microbial soil communities through PCR-DGGE of 16S rDNA gene fragments from a saline-alkaline soil. The soil and/or wastewater sludge were contaminated or not with phenanthrene and anthracene and spiked or not with acetone or polycyclic aromatic hydrocarbons (PAHs)-solution through an aerobic incubation during 56 days. The result obtained using denaturing gradient gel electrophoresis (DGGE) revealed that at the onset of the experiment, there were no differences between rybotipes in the nine treatments, but at 56 days, soil plus waste water sludge treatment showed the highest rybotipes number when spiked or not with acetone or PAH’s. Additionally, soil amended with wastewater sludge increased significantly the dissipation of phenanthrene and anthracene. It was found that soils amended with wastewater sludge increased the number of rybotipes and accelerated removal of phenanthrene and anthracene from soil.    


Key words: Microbial diversity, polycyclic aromatic hydrocarbons (PAHs) dissipation, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of ribosomal DNA, microbial communities, soil, sludge.