July 2013
Type E botulism associated with crude beef: A case report
Botulism remains infrequent but is widely feared because it can be severe and is potentially lethal. Foodborne botulism results from contaminated foodstuffs in whichClostridium botulinum spores have been allowed to germinate in anaerobic conditions. This typically occurs in home-canned food substances and fermented uncooked dishes. We present an outbreak of type E botulism in China. The diagnosis was...
July 2013
Properties of Enterococcus thailandicus isolates from beavers
Enterococcus thailandicus is characterized as the species of the genus Enterococcus, Family Enterococcacae, Order Lactobacillales, Class Bacilli, Phylum Firmicutes. This species novum is catalase-negative, chain forming coccus, and first detected from the fermented sausage (mum) in Thailand. Up to now, occurrence of enterococcal species from many animal sources was detected. Herbivor Castor...
July 2013
Effect of ambient storage on the microbial characteristics of traditional dried anchovies (Encrasicholina punctifer)
The aim of this study was to evaluate the safety of traditional dried anchovies by characterizing their microbial flora. Dried anchovies were stored at ambient temperature for three months and total aerobic bacterial (TAB), Staphylococcus aureus, Enterobacteriaceae, histidine decarboxylating bacterial (HDB), lysine decarboxylating bacterial (LDB) and ornithine decarboxylating bacterial (ODB) counts were...
July 2013
Biofilm, protease and lipase properties and antibiotic resistance profiles of staphylococci isolated from various foods
In this study, a total of 209 isolates were isolated from raw calf meat (minced), chicken drumsticks, raw milk, ice cream and white cheese samples. One hundred and seventy-five (83.7 %) coagulase-negative Staphylococci (CNS) and 34 (16.3%) coagulase-positive staphylococci (CPS) were isolated from these samples. The majority of Staphylococcusisolates showed biofilm formation (75.1%) and slime...
July 2013
A modified staining technique for the anatomical observation of mycorrhizal roots of woody trees
Modifications to the root staining method described by Phillips and Hayman (1970) were performed in order to develop a clear anatomical visualization of the mycorrhization status in avocado roots. Diverse KOH and H2O2 concentrations and various incubation times during clearing and acidification steps were assayed. The best results were obtained with the following treatment: once dried at 50°C for 12...
July 2013
Influence of straw degrading microbial compound on wheat straw decomposition and soil biological properties
The main challenge to successful use of wheat straw as soil amendment is its slow decomposition rate as compared to other crop residues. In order to solve this problem and increase farmers’ acceptability, inoculation of straw amended soil with microbial compounds to hasten decomposition is being promoted in China. In this experiment, the effect of three types of industrially produced composite microbial...
July 2013
Bacterial community composition of a saline-alkaline soil amended or not with wastewater sludge and contaminated with polycyclic aromatic hydrocarbons (PAHs)
The purpose of this study was to determinate the dynamics of the bacterial community composition, that is, diversity and abundance of microbial soil communities through PCR-DGGE of 16S rDNA gene fragments from a saline-alkaline soil. The soil and/or wastewater sludge were contaminated or not with phenanthrene and anthracene and spiked or not with acetone or polycyclic aromatic hydrocarbons (PAHs)-solution...
July 2013
Comparative effectiveness of different isolates of Azospirillum on nitrogen fixation and yield and yield attributing characters of tomato in Chhattisgarh
Among the total six isolates, isolates LAIS-21, LAIK-06 and LAIJ-04 were more potential regarding nitrogen fixation and fixed 19.2 mg, 14.9 mg and 11.0 mg N g-1 malate, respectively. Out of these three isolates, isolate LAIS-21 recorded higher plant height (45.5 cm), number of fruits per plant (36.2), fruit diameter (4.8 cm), fruit weight (57.9), root length (10.3 cm), fresh root weight (23.7 g), yield...
July 2013
Antimicrobial activity of aqueous extracts of sea cucumber (Isostichopus badionotus) from the coast of Yucatan, Mexico
The antibacterial activity of aqueous extracts from different tissues of Isostichopus badionotus was evaluated in this study. The antibacterial activity was detected in aqueous extracts of muscle and respiratory tract. A high antibacterial activity was found in the Membrane Protein fraction (MP) muscle...
July 2013
Antimicrobial susceptibility pattern of bacteria isolated from burn wounds in a tertiary care hospital in Pakistan
Burns constitute a major health concern with respect to morbidity and mortality; due to the risk of burn wounds infections caused by multi-drug resistant nosocomial pathogens. The present study was carried out to recover, identify the isolates, and to determine the antimicrobial susceptibility patterns of these pathogens from burn wounds in Khyber Teaching Hospital (KTH) Peshawar, during September 2007 to...
July 2013
Degradation kinetics of resorcinol by Enterobacter cloacae isolate
Resorcinol was utilized as the sole carbon and energy source by Enterobacter cloacae(identification by 16S rDNA nucleotide sequencing Genbank Accession Number JN093148). The different concentration of resorcinol utilized by the bacterial isolate ranged between 55 and 220 mg l-1 at 30°C and pH of 7.0. It was observed that the batch experimental results were best fitted for Michaelis-Menten...
July 2013
Characterization of six phosphate-dissolving bacteria isolated from rhizospheric soils in Mali
Maize rhizospheric soils in Mali were analysed for their content in microorganisms capable of dissolving phosphate rock and producing plant growth substances. Six bacteria were isolated and found to have the capacity to dissolve /solubilize the Tilemsi phosphate rock (TPR) by producing low molecular weight organic acids and phosphorus cycle enzymes. The bacterial isolates had an average of...
July 2013
Antimicrobial properties of the bacterial associates of the Arctic lichen Stereocaulon sp
Four bacterial associates were isolated from the Arctic lichen Stereocaulon sp. and identified based on their complete 16S rRNA gene sequences. Cell cultures were extracted using different solvents and tested for their antimicrobial properties using paper disk diffusion assay and MIC test against six test bacteria. Results show that the ethyl acetate of bacterial isolate is a most effective solvent...
July 2013
Identification of four major toxins of Clostridium perfringens recovered from clinical specimens
A simple polymerase chain reaction (PCR) procedure was used to identify four toxitypes of Clostridium perfringens collected from different origins. Eighteen (18) strains of C. perfringens were identified and typed by classical methods (dermonecrotic method in guinea pigs and sero-neutralization test in mice). All the strains were analyzed by PCR using gene of toxin alpha, gene of...
July 2013
Development of a cell line from the gill of koi and molecular characterization of KHV isolated in Taiwan
A new cell line (KoG) was developed from the gill of koi, Cyprinus carpio. KoG cells were composed of epithelial-like cells and fibroblast-like cells. The cells were maintained well at 25°C in Leibovitz L15 medium supplemented with 10% fetal calf serum. Chromosome analysis showed that the KoG cells had 43 diploid chromosomes at passage 40. The KoG cells were used to test the susceptibility of a koi...
July 2013
Effect of added nopal cactus (Opuntia ficus-indica) on microbial content in raw milk
Nopal cactus (Opuntia ficus-indica) represents an alternative for cattle feed during the drought season in the drier regions of Mexico. Previously, we have shown that feed with the addition of nopal reduced aerobic mesophiles (AM) and coliform (C) counts in milk. In order to determine whether the nopal additive has some direct effect on the microbiological quality of milk, nopal mucilage (NM), nopal epidermis...
August 2013
Isolation and polyphasic characterization of aerobic actinomycetes genera and species rarely encountered in clinical specimens
The aim of this study was to identify aerobic actinomycetes strains belonging to genera and species rarely encountered in infections. Clinical specimens (sputum, gastric fluid and abscess pus) are collected from patients with symptoms of tuberculosis, pneumopathy, septicemy or having abscess, hospitalized in different services of infectious diseases in Constantine University Hospital Center, East of Algeria....
July 2013
The role of ferrates (VI) as a disinfectant: Quantitative and qualitative evaluation for the inactivation of pathogenic bacteria
Iron compounds in the oxidation state (VI) have the advantage of being powerful antioxidants and bactericides, which explains their particular interest in water treatment.The aim of this work was to investigate the ability of inhibition of pathogenic microorganisms (Escherichia coli, Salmonella, Staphylococcus aureus, Bacillus sp., Pseudomonas sp., Enterococcus feacalis) by ferrate and for the...
July 2013
Comparison of OSOM BV Blue test with conventional methods for diagnosis of bacterial vaginosis
Bacterial vaginosis is the commonest cause of vaginal discharge in sexually active females. It is often associated with adverse pregnancy outcomes and increased susceptibility to other sexually transmitted diseases. In the present study, we evaluated efficacy of OSOM BV Blue test and compared it with conventional methods like Gram staining and Amsel’s criteria for diagnosing bacterial vaginosis. A total...
July 2013
Estimation of microbial flora in mince meat collected from Nandus nandus, Channa marulius and Puntius sarana during six months storage at -20°C
The study was designed to investigate the microbial estimation in the fish mince collected from Nandus nandus, Channa marulius and Puntius sarana which was stored at -20°C for a period of six months and analyzed every fortnightly for total plate count. During the study period, in the mince of N. nandus, C. marulius and P. sarana, there was a continuous decrease...
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