Effects of culture conditions and surfactants on marine lysozyme s-12 production using a statistical approach were studied in this work. Analysis of results were based on statistical calculations carried out with the one factor-at-a-time method and the L16-orthogonal array method, using MINITAB 15.0 software. All the fermentation runs were carried out at 30±2°C on a rotary orbital shaker at 200 rpm for 24 h. The individual components selected affected the fermentation process were CaCl2 > glucose >peptone > beef extract >MgSO4. Furthermore, Tween 80 with 0.4 mM could enhance the production of all the extracellular proteins and S-12. The final optimized medium supported S-12 activity of 1971.25 U/mL at the end of 24 h as compared with 1095.00 U/mL before optimization.
Key words: Lysozyme S-12, culture condition, surfactant, optimization, L16-orthogonal array.
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