African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Effects of culture conditions and surfactants on marine lysozyme S-12 production by Bacillus sp isolated from East China sea

Yuan Zheng, Lanhong Zheng, Jun Sheng, Xiaofeng Ji, Mi Sun*
Yellow Sea Fisheries Research Institute, Academic of Fishery Science of China, No. 106, Nan Jing Road, Qingdao, Shandong Province, 266071, P.R. China.
Email: [email protected]

  •  Accepted: 03 June 2013
  •  Published: 25 June 2013


Effects of culture conditions and surfactants on marine lysozyme s-12 production using a statistical approach were studied in this work. Analysis of results were based on statistical calculations carried out with the one factor-at-a-time method and the L16-orthogonal array method, using MINITAB 15.0 software. All the fermentation runs were carried out at 30±2°C on a rotary orbital shaker at 200 rpm for 24 h. The individual components selected affected the fermentation process were CaCl2 > glucose >peptone > beef extract >MgSO4. FurthermoreTween 80 with 0.4 mM could enhance the production of all the extracellular proteins and S-12. The final optimized medium supported S-12 activity of 1971.25 U/mL at the end of 24 h as compared with 1095.00 U/mL before optimization.


Key words: Lysozyme S-12, culture condition, surfactant, optimization, L16-orthogonal array.