Abstract

A proteomic approach was taken to compare the proteomes of EnteroinvasiveEscherichia coli (EIEC) infection alone, Lactobacillus plantarun pre-treatment and control group. Two-dimensional gel electrophoresis (2-DE), coupled with mass spectroscopy and protein database searching, 8 differentially expressed proteins was identified. Of them, Glutathione Transferase (down-regulated), Peroxisomal enoyl-coenzyme (up-regulated) and Peroxiredoxin (up-regulated) in EIEC infection group were compared with control group which are all associated with antioxidant-related proteins. Glyceraldehyde-3-phosphate dehydrogenase (up-regulated) and Triosephosphate Isomerase (up-regulated) were identified related with carbohydrate metabolism in EIEC infection group compared with control group. Keratin 8 (up-regulated) and hnRNP C1/C2 (down-regulated) were linked to antagonize cytoskeleton reorganization and apoptosis in L. plantarun pre-treatment group. Identification of these proteins provides insights that may lead to a better understanding of the molecular basis for EIEC infection process and L. plantarunprotection function.

Key words: Lactobacillus plantarun, Enteroinvasive Escherichia coli, tight junction.

Abbreviation

EIEC, enteroinvasive Escherichia coli; EPEC, enteropathogenic Escherichia coli.