African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Proteomic analysis of differentially expressed proteins in intestinal epithelial cell in response to Enteroinvasive Escherichia coli infection and Lactobacillus plantarun treatment

Zhongwei Zhang and Minghua Mao*
Department of Geriatrics, The 6th People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China.
Email: [email protected]

  •  Accepted: 27 October 2011
  •  Published: 30 December 2011


A proteomic approach was taken to compare the proteomes of EnteroinvasiveEscherichia coli (EIEC) infection alone, Lactobacillus plantarun pre-treatment and control group. Two-dimensional gel electrophoresis (2-DE), coupled with mass spectroscopy and protein database searching, 8 differentially expressed proteins was identified. Of them, Glutathione Transferase (down-regulated), Peroxisomal enoyl-coenzyme (up-regulated) and Peroxiredoxin (up-regulated) in EIEC infection group were compared with control group which are all associated with antioxidant-related proteins. Glyceraldehyde-3-phosphate dehydrogenase (up-regulated) and Triosephosphate Isomerase (up-regulated) were identified related with carbohydrate metabolism in EIEC infection group compared with control group. Keratin 8 (up-regulated) and hnRNP C1/C2 (down-regulated) were linked to antagonize cytoskeleton reorganization and apoptosis in L. plantarun pre-treatment group. Identification of these proteins provides insights that may lead to a better understanding of the molecular basis for EIEC infection process and L. plantarunprotection function.


Key words: Lactobacillus plantarun, Enteroinvasive Escherichia coli, tight junction.


EIEC, enteroinvasive Escherichia coli; EPEC, enteropathogenic Escherichia coli.