African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Prevalence, antibiotic resistance of Staphylococcus aureus isolated from layer farms (eggs, droppings/litter) in the outskirts of the city of Ouagadougou, Burkina Faso

Abdallah Sawadogo
  • Abdallah Sawadogo
  • Department of Biochemistry-Microbiology, Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
  • Google Scholar
Assèta Kagambèga
  • Assèta Kagambèga
  • Department of Biochemistry-Microbiology, Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
  • Google Scholar
Daniel Soro
  • Daniel Soro
  • Department of Biochemistry-Microbiology, Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
  • Google Scholar
Henseni Bognini
  • Henseni Bognini
  • Department of Biochemistry-Microbiology, Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
  • Google Scholar
Michel Dione
  • Michel Dione
  • International Livestock Research Institute, Dakar BP 24265, Senegal.
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Haoua Cissé
  • Haoua Cissé
  • Department of Biochemistry-Microbiology, Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
  • Google Scholar
Ibonyé Dieni
  • Ibonyé Dieni
  • Department of Biochemistry-Microbiology, Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
  • Google Scholar
Nicolas Barro
  • Nicolas Barro
  • Department of Biochemistry-Microbiology, Doctoral School of Science and Technology, Joseph KI-ZERBO University, Ouagadougou 03 BP 7021, Burkina Faso.
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  •  Received: 19 October 2023
  •  Accepted: 01 December 2023
  •  Published: 31 December 2023

Abstract

Eggs are susceptible to microbiological contamination by various pathogens on farms, particularly those belonging to the genus Staphylococcus, which are zoonotic bacteria capable of contaminating humans, animals, and/or birds, posing a significant public health concern. In Burkina Faso, limited data exists on the prevalence of Staphylococcus aureus, prompting this study to investigate the prevalence and antibiotic resistance of S. aureus on layer farms. To achieve this, a socio-demographic survey was conducted, followed by egg sampling from different farms. A pool of six eggs constituted one sample for each farm, and microbiological analysis was performed using standard methods. The antibiotic susceptibility of the strains was also tested. The results indicated a prevalence of 51.78% from litter, 35.59% from shells, and 10.16% from egg contents. The strains exhibited the highest resistance levels to Tetracycline (98%), Erythromycin (84%), Trimethoprim/Sulfamethoxazole (77%), Colistin (56%), Oxacillin (55%), and Cefoxitin (38%). However, the strains showed a high sensitivity to gentamycin (81%), and 56% were sensitive to Chloramphenicol. These findings highlight the contamination of eggs by antibiotic-resistant S. aureus, emphasizing the need to enhance good practices in the egg production chain to prevent such contamination.

 

Key words: Prevalence, antibiotic resistance, Staphylococcus aureus, poultry, Ouagadougou.