African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Comparison of clinical methods for the phenotypic detection of Methicillin resistant Staphylococcus aureus: Disc diffusion methods with BrillianceTM MRSA agar

Bashir Ahmad1*, Johar Jamil1 and Jawad Ahmed2
1Centre of Biotechnology and Microbiology University of Peshawar, Pakistan. 2Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan.
Email: [email protected]

  •  Accepted: 15 March 2013
  •  Published: 30 June 2013


This study was based on the comparison of revised guidelines of Clinical and Laboratory Standard Institute (CLSI), British Society for Antimicrobial Chemotherapy (BSAC) and BrillianceTM MRSA agar (Oxoid) for the phenotypic identification of methicillin resistantStaphylococcus aureus (MRSA). A total of 133 clinical isolates of S. aureus were tested using new CLSI, BSAC guidelines and BrillianceTM MRSA Agar. All the strains were tested according to prescribed guidelines of CLSI (M100-S20, 2010) and BSAC (10.2, 2011). The 30 µg and 10 µg cefoxitin (FOX) performed best for the detection of MRSA and gave 100% sensitivity and specificity. While 1 µg oxacillin (OX) showed less effective results showing sensitivity and specificity of 96.8% and 100% respectively. The cefoxitin disc diffusion method appears to be a good option for the identification of MRSA. BrillianceTM MRSA agar when used for the detection of MRSA, showed sensitivity and specificity of 98.4% and 100% respectively. In conclusion the efficiency of FOX is very high and FOX disc diffusion method is the most efficient method for the identification of MRSA phenotypicallyand could be a second choice for the detection of MRSA.


Key words: Staphylococcus aureus, CLSI, BSAC, Chromogenic media, MRSA.