African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Antioxidant and antibacterial activities of Camptotheca acuminate D. seed oil

Lin Wang1, 2, Zhiwei Yang1*, Sicen Wang3, Shuqiu Wang1 and Junxing Liu1*
  1School of Basic Medical Sciences, Jiamusi University, Jiamusi 15400, P. R. China. 2 The First Affiliated Hospital of Jiamusi University, Jiamusi 154003, P. R. China. 3Research and Engineering Center for Natural Medicine, Xi’an Jiaotong University, Xi’an 710061, P. R. China.
Email: [email protected], [email protected]

  •  Accepted: 11 November 2011
  •  Published: 30 December 2011



This study was designed to explore the in vitro antioxidant and antibacterial activities ofCamptotheca acuminate D. seed oil, which were extracted by supercritical fluid extraction (SFE) or petroleum ether extraction methods. The major constituent of the oil were described as (Z,Z,Z)-9,12,15-Octadecatrien-1-ol (54.92%) and 2-[(trimethylsilyl)oxy]-3-[4-[(trimethylsilyl)oxy]phenyl]-trimethylsilyl ester (26.53%) in supercritical fluid and petroleum ether extracts. The oil and the components were subjected to screen for their possible antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and β-carotene bleaching test. In the DPPH test system, free radical scavenging activities of supercritical fluid extracts and petroleum ether extracts were determined to be 7.55 ± 0.11% and 4.38 ± 0.08% (v/v), respectively. As to the β-carotene bleaching test system, the two values were 15.93 ± 0.11% and 6.87 ± 0.15% (v/v), respectively. The activities of antioxidant and antibacterial in components of petroleum ether were more efficient than in components of supercritical fluid extraction. As to the antimicrobial activities of the essential oil against 8 species bacterium, C. acuminate D. seed oil had remarkable antibacterial activity, especially to staphylococcus aureus (ATCC 6538). Thus, C. acuminate D. seed oil could be judged as a kind of patent drug which has antioxidant and antibacterial activity effectively.


Key words: Camptotheca acuminate D. seed oil, antioxidant activities, antibacterial activities.