African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Partial biochemical characterization of a thermostable chitinase produced by Streptomyces owasiensis isolated from lichens of the Amazonian region

Talita Camila Evaristo da Silva Nascimento
  • Talita Camila Evaristo da Silva Nascimento
  • Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n., CEP 52171-900, Recife, Pernambuco, Brazil
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José Erick Galindo Gomes
  • José Erick Galindo Gomes
  • Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n., CEP 52171-900, Recife, Pernambuco, Brazil
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Patrícia Lins Azevedo do Nascimento
  • Patrícia Lins Azevedo do Nascimento
  • Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n., CEP 52171-900, Recife, Pernambuco, Brazil
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Anna Carolina da Silva
  • Anna Carolina da Silva
  • Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n., CEP 52171-900, Recife, Pernambuco, Brazil
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Erika Valente de Medeiros
  • Erika Valente de Medeiros
  • Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Av. Bom Pastor, s/n, CEP 55292-270, Garanhuns, Pernambuco, Brazil
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Rosangela Estevão Falcão
  • Rosangela Estevão Falcão
  • Department of Biology, University of Pernambuco, Rua Capitão Pedro Rodrigues, 105, CEP 55294-902, São José, Garanhuns, Pernambuco, Brazil
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Maria Francisca Simas Teixeira
  • Maria Francisca Simas Teixeira
  • Department of Parasitology, Federal University of Amazonas, Av. General Rodrigo Octávio, 6200, CEP 69077-000, Coroado I, Manaus, Brazil
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Gláucia Manoella de Souza Lima
  • Gláucia Manoella de Souza Lima
  • Department of Antibiotics, Federal University of Pernambuco– UFPE, Av. Professor Moraes Rego, 1235, CEP 506070–901 Cidade Universitária, Recife, Pernambuco, Brazil
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Ana Lúcia Figueiredo Porto
  • Ana Lúcia Figueiredo Porto
  • Department of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros, s/n., CEP 52171-900, Recife, Pernambuco, Brazil
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Keila Aparecida Moreira*
  • Keila Aparecida Moreira*
  • Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Av. Bom Pastor, s/n, CEP 55292-270, Garanhuns, Pernambuco, Brazil
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  •  Received: 07 April 2014
  •  Accepted: 07 July 2014
  •  Published: 23 July 2014

Abstract

The current study aims to identify Streptomyces spp., isolated in the Amazon region and capable of producing chitinase as well as to partially characterize the enzyme. Optimum temperature and pH, thermal and pH stabilities and the behavior of chitinase were determined as compared to other substances. Streptomyces owasiensis was the best chitinase producer in submerged culture fermentation using chitin 1% (w/v) at 140 rpm for 96 h at 34°C. The enzyme showed optimal activity at pH 7.0 and stability at the assessed pH variations. It also showed optimal temperature of 80°C and 180 min of thermostability between 30 and 90°C. The enzymatic activity was potentiated in the presence of various ions, especially Fe2+, the same occurred in the presence of the anionic surfactant sodium dodecyl sulfate (SDS), but it suffered inhibition influence by EDTA. The chitinase produced by S. owasiensis showed characteristics of industrial relevance and it highlights the first report on enzyme production from the species isolated in the Amazon.

Key words: N-acetyl-D-glucosamine, chitin, chitinase, actinomycetes, Streptomyces owasiensis.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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