African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Effect of growth conditions on glutathione accumulation, gshR gene expression and resistance to the lyophilization process in Lactococcus lactis LVA. 2

Eliana dos Santos Leandro*
  • Eliana dos Santos Leandro*
  • Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil
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Graciela Kunrath Lima
  • Graciela Kunrath Lima
  • Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, 31270-901, Brazil
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Antônio Fernandes de Carvalho
  • Antônio Fernandes de Carvalho
  • Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil
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Poliane Alfenas Zerbini
  • Poliane Alfenas Zerbini
  • Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil
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Odilon Gomes Pereira
  • Odilon Gomes Pereira
  • Departamento de Zootecnia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil
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Célia Alencar de Moraes
  • Célia Alencar de Moraes
  • Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, 36570-000, Brazil
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  •  Received: 12 January 2014
  •  Accepted: 27 June 2014
  •  Published: 23 July 2014

Abstract

The functionality of glutathione (GSH) and the effect of growth conditions on GSH accumulation, gshR gene expression and viability following lyophilization were examined in Lactococcus lactis LVA. 2. The accumulation of GSH under anaerobic and continuously stirred aerobic conditions enabled a higher GSH accumulation than a static aerobic culture, which is the growth condition normally used. The gshR gene expression under the growth conditions in the anaerobic, static aerobic and continuously stirred aerobic media was similar. Although, GSH failed to provide an apparent protection to L. lactis LVA. 2 following the lyophilization process, the fact that this strain accumulates GSH and expresses the gshR gene makes it a promising strain to cope with other stress situations.

Key words: Lactococcus lactis, reduced glutathione, lyophilization, survival. 

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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