African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5229

Full Length Research Paper

Analysis of the RNAi targeting FAD2 gene on oleic acid composition in transgenic plants of Brassica napus

  Tian Baoming1, Sun Dandan1, Lian Yuli2, Shu Haiyan1, Ling Hua1, Zang Xin1, Wang Bonan1 and Pei Zhenqiang1
  1Bioengineering Department, Zhengzhou University, Zhengzhou, 450001, China. 2Chaoyang Teachers College, Chaoyang, 122500, China.
Email: [email protected]

  •  Accepted: 01 March 2011
  •  Published: 30 April 2011

Abstract

 

Oleate-Δ12 desaturase (FAD2) is a key enzyme involved in the conversion of oleic acid (C18:1) into linoleic acid (C18:2). Brassica napus FAD2 gene was targeted for silencing by its RNAi gene under the control of a seed-specific napin promoter. This study aims to identify the transgenic plants, and analyze the level of BnFAD2 transcripts in addition to fatty acid profile in T3 seeds. As indicated by PCR and southern blotting analysis, a total of six transgenic plants were developed. Analysis performed by RT-PCR revealed the significant down-regulation of BnFAD2 transcripts in developing T3 seeds, which resulted in 13.90 to 32.20% increase of oleic acid composition in mature T3 seeds. The data demonstrated that BnFAD2 gene was efficiently down-regulated and mediated by its RNAigene, and oleic acid composition in transgenic rapeseeds was significantly enhanced.

 

Key words: RNAi, oleate-Δ12 desaturase (FAD2) gene, oleic acid, Brassica napus.