African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Microbial biodegradation of butachlor pollution (obsolete pesticide Machete 60% EC)

Sherif H. Abd-Alrahman1,2* and Mounir M. Salem-Bekhit3,4
1Biomarkers Research Program, Department of Biochemistry, College of Science, King Saud University, P. O. Box, 2455, Riyadh, 11451, Saudi Arabia. 2Central Agricultural Pesticide Laboratory, Pesticides Residue and Environmental Pollution Department, Agricultural Research Center, Giza 12618, Egypt. 3Department of Pharmaceutics, College of Pharmacy, King Saud University, P. O. Box 2457, Riyadh, Saudi Arabia. 4Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
Email: [email protected]

  •  Accepted: 20 December 2012
  •  Published: 31 January 2013


Accumulation of obsolete pesticides in the environment is a global problem. The scale of the problem in different countries varies depending on economic situation and social awareness. Only limited data are available on the microbial biodegradation of butachlor. Biodegradation of butachlor by different microorganisms was investigated. Six bacterial strains were isolated from an agricultural soil and found to be actively utilized butachlor, as a sole source of carbon and energy. Based on their morphological and biochemical categorization, the six bacterial and fungal isolates were identified as Psedomonas alcaligensBacillus licheniformisBacillus megateriumTrichoderma virideRhizobium huakuii and Bradyrhizobium japonicum. Results show that the Trichoderma viride andPsedomonas alcaligens quickly degraded butachlor and reached nearly 98 and 75% in a medium containing 50 mg/kg of butachlor after 15 and 21 days, respectively. Our results can conclude that these two organisms can be usedto degrade the obsolete butachlor formulation.


Key words: Obsolete pesticides, herbicide, butachlor, biodegradation