African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Parametric optimization of extracellular α-amylase recovery from the fermented bran of Aspergillus awamori MTCC 9997

Kalaiarasi K.1* and Parvatham R.2
1Department of Textiles and Clothing, Avinashilingam University for Women, Coimbatore-43, Tamil Nadu, India. 2Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam University for Women, Coimbatore-43, Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 30 June 2011
  •  Published: 09 September 2011


A newly isolated Aspergillus awamori MTCC 9997 strain from spoiled cooked rice was cultured under solid state using cassava peel powder to produce α-amylase. The effect of extraction medium, solid to solvent ratio, extraction time, temperature and physical state on α-amylase recovery was examined. Among various organic, inorganic solvents and buffer solutions tested, maximum extraction of α-amylase was achieved when phosphate buffer of pH 7.0 was added. A solid to solvent ratio (cassava peel : phosphate buffer) of 1:10 (w/v) resulted in maximum yield of α-amylase. The optimum extraction time was 30 min. At 30°C, α-amylase recovery was found to be the maximum. An appreciable amount of α-amylase was recovered under agitated conditions of the fermented biomass at 150 rpm compared with the quantum recovered under stationary conditions.


Key words: α-Amylase, Aspergillus awamori, solid-state fermentation, extraction.