African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Isolation, classification, phylogenetic analysis and scanning electron microscopy of halophilic, halotolerant and alkaliphilic actinomycetes isolated from hypersaline soil

Ismet Ara1,2*, D. Daram4, T. Baljinova4, H. Yamamura5, W. N. Hozzein3, M. A. Bakir1, M. Suto2 and K. Ando2
1Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 22452, Riyadh-11495, Kingdom of Saudi Arabia. 2Biotechnology Development Center, Department of Biotechnology (DOB), National Institute of Technology and Evaluation (NITE), Kazusakamatari 2-5-8, Kisarazu, Chiba, 292-0818, Japan. 3Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia. 4Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan
Email: [email protected], [email protected]

  •  Accepted: 09 January 2013
  •  Published: 22 January 2013


Actinomycetes were isolated by plating of serially diluted samples onto humic acid-vitamin agar prepared with or without NaCl and characterized by physiological and phylogenetical studies. A total of 16 strains were isolated from hypersaline soil in Mongolia. All strains showed alkaliphilic characteristics and were able to abundantly grow in media with pH 9.0. Six strains were halotolerant actinomycetes (0 to 12.0% NaCl) and 10 strains were moderately halophiles (3.0 to 12.0% NaCl). Most strains required moderately high salt (5.0 to 10.0%) for optimal growth but were able to grow at lower NaCl concentrations. Among the 16 strains, 5 were able to grow at 45°C. Among the group of moderate halophilic actinomycetes, 9 strains were phylogenetically detected under the genus Nocardiopsis in the family Nocardiopsaceae. Results of scanning electron microscopic study demonstrated that most of the moderately halophilic strains under the genusNocardiopsis, produce synnemata structure. Overall phylogenetic analysis based on 16S rRNA gene sequences analysis revealed that all the 16 strains fell into 4 different genera:Nocardiopsis (9 strains), Isoptericola, (2 strains), Nesterenkonia (2 strains),Streptomyces (3 strains). This preliminary study demonstrated that the genusNocardiopsis is abundant and a recoverable actinomycetes group in Mongolian saline soil. Based on the position in phylogenetic tree of 16 isolates, there were new clusters which supposed to be novel species under the genera Nocardiopsis (possible new species 1) and Isoptericola (possible new species 1) in the family Nocardiopsaceae andPromicromonosporaceae, respectively.


Key words: Classification, moderate halophilic and halotolerant actinomycetes, hypersaline soil, phylogenetic analysis, scanning electron microscope