African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Biological study of hypertrophy sorosis scleroteniosis and its molecular characterization based on LSU rRNA

Ruihua LÜ, Aichun ZHAO, Jun LI, Xiling WANG, Yasheng YU, Cheng LU and Maode YU*
The State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, 400716,  People's Republic of China.
Email: [email protected]

  •  Accepted: 03 May 2012
  •  Published: 25 June 2013


During this work, the pathogen agent of hypertrophy sorosis scleroteniosis was cultivated on artificial culture, and based on the morphological characteristics, the causal agent was identified as Sclerotinia sclerotiorum. The amplification of large subunit ribosomal RNA (LSU rRNA) was done, furthermore LSU rRNA was sequenced. Result shows that the molecular weight was 612 bp, and compared with the known LSU rRNAs sequence of different S. sclerotiorum strains found from NCBI database. On the molecular level, the pathogen agent of hypertrophy sorosis scleroteniosis was S. sclerotiorum. Biological characteristics showed that S. sclerotiorum mycelia can produce oxalic acid during the growth process, but they did not show any sclerotia form in liquid culture.


Key words: Apothecia, hyphae, hypertrophy sorosis scleroteniosis, Sclerotinia sclerotiorum.