African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

The influence of carbon and nitrogen supplementation on alpha amylase productivity of Bacillus amyloliquefaciens IIB-14 using fuzzy-logic and two-factorial designs

Mian Sahib Zar1,2*, Sikander Ali2 and Ahmad Ali Shahid1
This study is concerned with the production and optimization of alpha amylase by Bacillus amyloliquefaciens IIB-14 in the presence of additional carbon and nitrogen sources using solid state fermentation. Alpha amylase is of special importance in the starch fermentation industry, as it has significant commercial applications in starch processing, beverages, foods, bread and baking, medicines, textile, paper, pharmaceuticals, sugar and detergent industries. Maltose, glucose, lactose and soluble starch were supplemented as carbon sources. Urea, peptone, yeast extract and nutrient broth as organic nitrogen sources while NH4NO3, (NH4)2SO4, NaNO3 and KNO3 as inorganic nitrogen sources were evaluated. Among them, a marked improvement in enzyme production (70.15 U/mg/min) was obtained with lactose, yeast extract and NH4NO3 at a level of 1.5, 1 and 1%, respectively. The performance of fuzzy-logic system control was found to be highly promising for the improved substrate conversion rate of more than 75%. The process parameters were further identified using Plackett-Burman design. The correlation (1.045E+0025) among variables demonstrated the model terms as highly significant (HS) indicating commercial utility of the culture used (p<0.05). The maximal enzyme activity (84.24 U/mg/min) of crude enzyme was found at pH 7 and temperature 70°C incubated for 30 min.   Key words: Alpha amylase, Bacillus amyloliquefaciens, solid state culture, carbon and nitrogen supplementation, fuzzy-logic control, two-factorial experimental design
Email: [email protected]

  •  Accepted: 08 January 2013
  •  Published: 31 January 2013

Abstract

This study is concerned with the production and optimization of alpha amylase by Bacillus amyloliquefaciens IIB-14 in the presence of additional carbon and nitrogen sources using solid state fermentation. Alpha amylase is of special importance in the starch fermentation industry, as it has significant commercial applications in starch processing, beverages, foods, bread and baking, medicines, textile, paper, pharmaceuticals, sugar and detergent industries. Maltose, glucose, lactose and soluble starch were supplemented as carbon sources. Urea, peptone, yeast extract and nutrient broth as organic nitrogen sources while NH4NO3, (NH4)2SO4, NaNOand KNO3 as inorganic nitrogen sources were evaluated. Among them, a marked improvement in enzyme production (70.15 U/mg/min) was obtained with lactose, yeast extract and NH4NOat a level of 1.5, 1 and 1%, respectively. The performance of fuzzy-logic system control was found to be highly promising for the improved substrate conversion rate of more than 75%. The process parameters were further identified using Plackett-Burman design. The correlation (1.045E+0025) among variables demonstrated the model terms as highly significant (HS) indicating commercial utility of the culture used (p<0.05). The maximal enzyme activity (84.24 U/mg/min) of crude enzyme was found at pH 7 and temperature 70°C incubated for 30 min.

 

Key words: Alpha amylase, Bacillus amyloliquefaciens, solid state culture, carbon and nitrogen supplementation, fuzzy-logic control, two-factorial experimental design