Abstract

A strain of hydrocarbon degrading yeast named YH-41 was isolated and purified from the soil polluted by crude oil from Shengli oil field, China. The strain was identified by morphological, biochemical characteristics and 18S rDNA sequence analysis, the results show that the strain was similar to the haploid nucleus of Pichia ohmen, which belong toCandida., The GC analysis showed that the yeasts degradated diesel oil. Through bioremediation experiments, we can study the feasibility of bioremediation of oil-polluted soil by yeasts. The results show that the haploid nucleus of P. ohmen has a certain role on accelerating oil degradation in soil early in the test. The degradation rate of residual oil in the same period of time increased about 20% compared with other treatment without adding the yeasts.

Key words: 18S rDNA, Candida, Pichia ohmen, haploid, GC analysis and bioremediation