Abstract

The accelerated solvent extraction method was used to extract metabolites from marine fungi and the process parameters were optimized. The influences of the times of extraction, temperature, pressure and extraction time duration on the extraction yield were discussed using single-factor experiment. The optimum process parameters were then obtained using orthogonal experiment. The optimal conditions for accelerated solvent extraction of metabolites from marine fungi were obtained: extracted for 2 times with methanol and ethyl acetate (80:20, V/V), extraction pressure of 1.2 × 10⁷ Pa, extraction temperature of 105°C, and static extraction time of 15 min. Under the optimum conditions, the extraction yield was much higher for accelerated solvent extraction method when compared with soaking method, whereas, the accelerated solvent extraction method took only 1/3 of time for soaking method and saved over 80% of extraction solvent. The results showed that accelerated solvent extraction method was suitable for extracting metabolites from marine fungi in batch.

Key words: Accelerated solvent extraction, high-performance, liquid chromatography, marine fungi, solid fermentation.