Full Length Research Paper
Abstract
In this study, sequencing and phylogenetic analysis of partial sequences of the gene that encodes alpha toxin from field and vaccine strains of Clostridium septicum from Brazil were performed to analyze genetic differences in these strains. Alpha toxin nucleotide and amino acid sequences from five field strains of C. septicum and four strains used in the production of vaccines against clostridiosis were compared. Based on this sequence analysis, the C. septicum strains can be classified in two groups. Group 1 presents the majority of the sequences; including two vaccine strains and is very conserved. Group 2 presents sequences with significative number of changes in the nucleotide and amino acid sequences, and contains the other two vaccine strains used in the production of vaccines in Brazil. Therefore, genetic diversity occurs in Brazilian C. septicum strains.
Key words: Clostridium septicum, alpha toxin, sequencing, phylogenetic analysis.
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