Evaluation of culture media for selective enumeration of Bifidobacterium spp. in combination with different strains of Streptococcus thermophilus isolated from commercial yogurt starter cultures

Luciana Rodrigues Faleiro; Beatriz Silva Pereira; Inayara Cristina Lacerda; Jacques Robert Nicoli; Tassia Costa Souza; Alvaro Cantini Nunes; Luige Biciati Alvim; Marcelo Resende de Souza; Evelyn de Souza Oliveira*

doi:10.5897/AJMR2014.7269

African Journal of
Microbiology Research

Abbreviation: Afr. J. Microbiol. Res.
Language: English
ISSN: 1996-0808
DOI: 10.5897/AJMR
Start Year: 2007
Published Articles: 5228

Full Length Research Paper

Evaluation of culture media for selective enumeration of Bifidobacterium spp. in combination with different strains of Streptococcus thermophilus isolated from commercial yogurt starter cultures

Luciana Rodrigues Faleiro

Luciana Rodrigues Faleiro
Departamento de Alimentos, Faculdade de Farmacia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Beatriz Silva Pereira

Beatriz Silva Pereira
Departamento de Alimentos, Faculdade de Farmacia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Inayara Cristina Lacerda

Inayara Cristina Lacerda
Departamento de Alimentos, Faculdade de Farmacia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Jacques Robert Nicoli

Jacques Robert Nicoli
Departamento de Microbiologia, Instituto de Ciencias Biologicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Tassia Costa Souza

Tassia Costa Souza
Departamento de Microbiologia, Instituto de Ciencias Biologicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Alvaro Cantini Nunes

Alvaro Cantini Nunes
Departamento de Microbiologia, Instituto de Ciencias Biologicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Luige Biciati Alvim

Luige Biciati Alvim
Departamento de Microbiologia, Instituto de Ciencias Biologicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Marcelo Resende de Souza

Marcelo Resende de Souza
Departamento de Tecnologia e Inspecao de Produtos de Origem Animal, Escola de Veterinaria, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Evelyn de Souza Oliveira*

Evelyn de Souza Oliveira*
Departamento de Alimentos, Faculdade de Farmacia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil
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Article Number - DD0DF8550562
Vol.9(6), pp. 373-281 , February 2015
https://doi.org/10.5897/AJMR2014.7269

Received: 13 November 2014
Accepted: 02 February 2015
Published: 11 February 2015

Abstract

Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus are involved in the manufacture of yogurt, and Bifidobacterium spp. can be supplemented if probiotic properties are expected. In such products, enumeration of each bacterial component is a hard task since they have similar nutritional requirements. For this reason, there is still no official and reliable methodology for this purpose. The objectives of the present study were to evaluate the most appropriate culture medium for the enumeration of four different Bifidobacterium species in the presence of S. thermophilus and L. delbrueckii subsp. bulgaricus isolated from commercial cultures and investigate the influence of different strains of S. thermophilus on the choice of medium. Among 11 isolates of S. thermophilus obtained from these cultures, and which were submitted to repetitive element sequence-based PCR (rep-PCR), nine were found to be distinct strains. For selective enumeration of the Bifidobacterium species, results were influenced by the S. thermophilus strain used, but De Man, Rogosa and Sharpe (MRS) agar containing bile salts showed the best results for all of the Bifidobacterium species.