African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Detection of H9N2 avian influenza virus in various organs of experimentally infected chickens

Somayeh Asadzadeh Manjili1, Iradj sohrabi Haghdoost1, Pejman Mortazavi1, Hamid Habibi2, Hadi lashini3 and Esmaeil Saberfar3*
  ¹Department of veterinary pathology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Researches Branch, Tehran, Iran. ²Department of Veterinary, Saveh, Markazi Province, Iran. 3Research Center for applied virology, Baqiyatallah University of Medical Sciences Tehran, IR Iran.
Email: [email protected]

  •  Accepted: 30 September 2011
  •  Published: 30 December 2011



H9N2 Avian influenza virus (AVI) infection is a major cause of economic losses in poultry industry. Therefore further study to explain the virus pathogenesis is necessary. In this study tissue tropism and dissemination of A/chicken/Iran/11T/99(H9N2) virus in various organs of specific pathogen free (SPF) chickens were investigated. Fifty 2-week-old chickens hatched from SPF eggs were divided randomly into two groups. Forty chicks in the experimental and ten chicks in the control group. Experimental chicks were inoculated intranasally-intraorally with the virus. Samples of lung, trachea, pancreas, thymus, spleen, brain, bursa of fabricius, proventriclus, cloaca and kidney were aseptically collected at 1, 3, 5, 7, 9 and 10 day post inoculation (DPI). A reverse transcriptase polymerase chain Reaction (RT-PCR) test was performed for virus detection. Viral RNA was detected in the respiratory system on days 3, 5 and 7 PI. The virus was also found in the kidney on days 3,5,7,9 PI and in the pancreas on days 3 and 5 PI. Viral RNA was observed only on day 5 PI in cloaca. The virus was not detected in the blood, brain and immune system. The virus was not found from any organs on day 10 PI. These results suggest that H9N2 AIV has tropism for respiratory, digestive and urinary system following intranasal/intraoral inoculation.


Key words: Avian influenza, H9N2, SPF, RT-PCR.