African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Production and characterization of a thermostable beta-propeller phytase from Bacillus licheniformis

Yumao Wang1, Yichun Wu2, Jing Han3, Shijun Fu1,2, Shijin Guo1 and Zhiqiang Shen1,2,3*
1Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, People’s Republic of China. 2Biological Engineering College of Binzhou Polytechnic, Binzhou 256600, People’s Republic of China. 3Binzhou Preferential Hospital, Binzhou 256600, People’s Republic of China.
Email: [email protected]

  •  Accepted: 25 March 2013
  •  Published: 23 April 2013


A novel beta-propeller phytase producing thermophilic strain of Bacillus licheniformis was isolated. The culture conditions for production of phytase by B. licheniformis under shake flask culture were optimized to obtain high levels of phytase (0.267 U/ml). The optimum temperature and pH of the phytase from B. licheniformis (PhyC) were 55°C and 7.0, respectively. The enzyme exhibited good thermostability and pH stability in the presence of 1 mM CaCl2After treatment at 80°C, pH 7.0 for 10 min, the residual activity of PhyC was 57.36%. Over 80% of PhyC activity was retained after treatment by preincubation over a pH range of 6.5 to 9.0 for 1 h at 25°C. Substrate was very specific for sodium phytate and showed no activity on other phosphate esters. Its activity was greatly inhibited by EDTA and metal ions such as Cd2+, Mn2+, Cu2+ and Ba2+.


Key words: Bacillus licheniformis, phytase, production, optimization, characterization.