African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Molecular characterization and phylogenetic study of Newcastle disease virus isolates from the 2010 to 2011 outbreaks in Shiraz, Iran

Ali Reza Boostani1* Seyed Ali Pourbakhsh2, Reza Momayez2 and Saied Charkhkar3  
1Department of Microbiology Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. 2Department of Research and Diagnosis of  Poultry Diseases, Razi Vaccine and Serum Research Institute Karaj,  Iran. 3Department of Poultry Diseases, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Email: [email protected]

  •  Accepted: 22 November 2012
  •  Published: 19 February 2013


The velogenic Newcastle disease virus (NDV) causes a devastating form of disease in commercial poultry. In this study, we characterized 10 isolates of NDVs that were obtained from recent outbreaks in commercial broiler chickens in Shiraz (in the Southwest of Iran) during 2010 and 2011. The F protein gene was amplified by RT-PCR using kit with F gene specific primers. A piece of 1561 nucleotide including F gene cleavage site was sequenced. All isolates had 112R-R-Q-K-R-F117 sequences at the cleavage site in the F protein genes, typical of virulent NDV strains. Phylogenetically compared with previous viruses in Iran and some world strains, it was found that these isolates clearly separated from earlier isolates of Iran and clustered with some Chinese isolates. Phylogenitically, distance relationship of NDVs of our study with other Iranian strains indicates that there are new strains of NDV now present in Iran.


Key words: Newcastle disease virus, F gene, Cleavage site, RT-PCR, Phylogenetic analysis, Shiraz, Iran