African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Resistant virulent Candida species colonizing preterm neonates and in vitro promising prospect of chlorhexidine gluconate

Iman E. Wali1*, Rasha H. Bassyouni2, Eman Ahmed El-Seidi1, Amira Edris3, Abdel-Rahman Ahmed Abdel-Razek3, Amina Abdel-Salam3 and Reem Fouad4
1Medical Microbiology and Immunology Department, Faculty of Medicine, Cairo University,Egypt. 2Medical Microbiology and Immunology Department, Faculty of Medicine, Fayoum University, Egypt. 3Department of Pediatrics, New Children’s Hospital, Cairo University, Egypt. 4The Ministry of Health, Cairo, Egypt.
Email: [email protected]

  •  Accepted: 12 June 2013
  •  Published: 25 June 2013


The present study aimed to investigate the potential virulence factors and antifungal resistance of 31 Candida albicans and 21 non-albicans Candida isolates colonizing preterm neonates. The study also compared the susceptibility results with the in vitroactivity of chlorhexidine in the eradication of Candida colonization.  Candida albicansproduced significantly more phospholipase and coagulase than non-albicans Candida, whereas proteinase production was higher in non-albicans Candida.  Biofilm production was demonstrated in Candida albicans and non-albicans Candida (= 0.214). None of the planktonic growth of Candida isolates were resistant to either fluconazole or amphotericin B, whereas 40% and 84% Candida isolates grown as biofilm became resistant to fluconazole and amphotericin B, respectively. Both coagulase and phospholipase production  strongly correlated with the resistance of sessile Candidaisolates to amphotericin B (< 0.001). Whereas both proteinase and phospholipase correlated with the resistance of in vitro Candida biofilms to fluconazole (P < 0.05 and = 0.001; respectively). Chlorhexidine was comparable to fluconazole towards planktonic and sessile grown Candida isolates. In conclusion, the study demonstrated an association between certain virulence factors and the development of biofilm drug resistance and highlighted the value of chlorhexidine as a promising prospect in the eradication ofCandida colonization.


Key words: Antifungal susceptibility, biofilm resistanceCandida colonization,chlorhexidine, preterm neonates, virulence factors.