African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Table of Content: 16 April, 2013; 7(16)

April 2013

Advances in nutritional manipulation rumen functions of surfactants

Surfactant, including chemical surface active agent and biosurfactants, as a new type offeed additive applied in ruminant production, can effectively improve the degradability of roughages in the rumen and productivity of ruminants by increasing the emulsification of ruminal liquid, the number of ruminal microorganisms and the activity of ruminal endogenous enzymes secreted by ruminal microbes; and changing the...

Author(s): Y. Liu,, L.M.Camacho, S. Tang, Z.Tan, and A.Z.M. Salem,

April 2013

Tn5 siderophore producing mutants of Rhizobium and its role in nitrogen fixation and iron uptake in pigeonpea

Pigeonpea is an economically important kharif grain legume crop that nodulates poorly in the northern part of India. The role of siderophore production in nitrogen fixation and iron uptake in pigeonpea (Cajanus cajan) was assessed. 25 Tn5 siderophore producing mutants of pigeonpea-Rhizobium strain PP-18 were selected using Chrome Azurol S (CAS) agar plate as well as CAS assay solution. Finally, the efficacy in...

Author(s): Joginder Singh Duhan

April 2013

Screening and characterization of native Pseudomonas sp. as plant growth promoting rhizobacteria in chickpea (Cicer arietinum L.) rhizosphere

The search for diverse plant growth promoting rhizobacteria (PGPR) is gaining momentum as efforts are directed to exploit them as low-input biotechnology forsustainable agriculture. In search of efficient PGPR (Pseudomonas sp.) with multiple plant growth promoting (PGP) activities, a total of 35 isolates of rhizobacteria were isolated from 25 soil samples collected from healthy chickpea rhizospheric locations...

Author(s): Navprabhjot Kaur and Poonam Sharma

April 2013

Detection of intestinal microflora from mice affected by macrolide antibiotics

 Macrolide antibiotics are widely used for treatment of community-acquired infectious diseases, but its side effects have not been thoroughly investigated, especially on the intestinal tract. Erythromycin, roxithromycin and azithromycin are macrolide antibiotics sharing similar chemical structure and their side effects on intestinal microflora of BALB/c mice were tested in this study. The bacterial...

Author(s): Li Xin-Li, Liu Jian-jun, Wu Da-Chang, Zhang Cui-Li and Xin Yi

April 2013

Population, morphological and chemotaxonomical characterization of diverse rare actinomycetes in the mangrove and medicinal plant rhizosphere

Actinomycetes populations in rhizosphere soils of mangrove forests in Cox’s Bazar and medicinal plant in Dhaka, Bangladesh were examined by simple dilution and an agar plate method. Actinomycetes populations (colony forming units/g, soil samples) ranged from 1x103 to 157x103 among 20 mangrove rhizosphere soil samples and 22x103 to 168x103in 12 medicinal plant rhizosphere soil samples of Bangladesh....

Author(s): Ismet Ara, H. Al. Wathnani and T. Kudo

April 2013

Chemical composition and antimicrobial activity of essential oil of Salvia potentillifolia Boiss. & Heldr. ex Benth. from Turkey

 The present study describes the chemical composition and antimicrobial activity of essential oil from Salvia potentillifolia Boiss. & Heldr. ex Benth., which is endemic for Turkey. The essential oil was obtained by hydrodistillation and components of the essential oil were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The major components of S....

Author(s): Elif OdabaÅŸ Köse, Gözde Öngüt and Atila YanıkoÄŸlu

April 2013

Isolation, characterization, and antimicrobial activity of endophytic bacteria from Polygonum cuspidatum

Polygonum cuspidatum Sieb. et Zucc. is a traditional Chinese medicinal plant that produces polydatin, a glycosylated derivative of resveratrol. Thus far, 244 prokaryotic endophytes have been isolated and identified from P. cuspidatum using 16s rDNA sequence. The results show that the endophytic bacteria in P. cuspidatum belong to five orders,...

Author(s): Hui Sun, Yan He, Qing Xiao, Renyuan Ye, and Yongqiang Tian,

April 2013

Detection of Toxoplasma gondii in environmental matrices (water, soil, fruits and vegetables)

          Toxoplasmosis is a widely distributed protozoan disease caused by Toxoplasma gondii. The infective stages of T. gondii are capable of infecting a variety of vertebrates including humans. Domestic and wild felids are capable of serving as definitive hosts and T. gondiioocysts are excreted in their faeces. Toxoplasmosis is caused by...

Author(s): Adeela Ajmal, Azhar Maqbool, Muhammad Fiaz Qamar, Kamran Ashraf and Aftab Ahmad Anjum

April 2013

Chemical and microbiological quality of Anatolian Buffalo milk

This study was carried out to determine the chemical and microbiological quality of Anatolian buffalo milk. A total of 120 Anatolian buffalo raw milk samples were collected monthly from Afyonkarahisar province, throughout the year for this study. In the chemical analysis of Anatolian buffalo milk samples the mean total solid value, non-solid fat, lipid, protein, lactose,...

Author(s): Zeki Gürler, Yahya KuyucuoÄŸlu and Åžebnem Pamuk

April 2013

Frequency of canine leptospirosis in dog shelters in Veracruz, Mexico

A cross-sectional study was conducted to determine the frequency of canine leptospirosis in 92 dogs from two shelters in the city of Veracruz, Mexico. The microaglutination test (MAT) was used for detecting antibodies against 12 serovars of Leptospira interrogans in dog serum. Of the 92 dogs, eight were positive, resulting in an overall frequency of leptospirosis of 8.6% (CI95% 3.8 to 16.41)....

Author(s): Anabel Cruz-Romero, Dora Romero-Salas, Concepción Ahuja Aguirre, Mariel Aguilar-Domínguez and Christian Bautista-Piña

April 2013

Comparative contamination of Listeria monocytogenes in traditional dairy products in Esfahan Province, Iran

Listeria monocytogenes, a primary human pathogen, has been found in different places in the environment of dairy, and the bacterium may survive for a long time in a dairy. L. monocytogenes is a major concern for the food industry, as it can cause listeriosis in humans. Listeriosis is one of the most important infections in Europe and the United States. It may cause fever, muscle aches and gastroenteritis;...

Author(s): Amir Masoud Shahbazi, Mojtaba Rashedi and Rafei Sohrabi

April 2013

High nitrite removal capacity of an aerobic denitrifier Klebsiella oxytoca DF-1 isolated from aquaculture ponds in coastal mudflats

The aims of this study were to help the development of the models describing denitrification in the recirculation aquaculture systems and to contribute to the knowledge on the role of heterotrophic bacteria in aquaculture nutrient removal systems in mudflats along the coast. Six aerobic denitrifying strains were isolated by bromothymol blue (BTB) plate technique from the fish farm sediments in mudflat along...

Author(s): Yuanrui Liu, Yang Zang, Xiaohua Long, Hongbo Shao,, Zhaopu Liu, Zhenhua Zhang and Zed Rengel

April 2013

Enhancement of the thermo-alkali-stability of xylanase II from Aspergillus usamii E001 by site-directed mutagenesis

Replacing several serine and threonine residues on the Ser/Thr surface of xylanase II (Xyn II) from Aspergillus usamii E001 with arginines effectively increased the thermostability of the enzyme. The substitution of Ser and Thr residues on the Ser/Thr surface of the enzyme with four (ST4) or five arginines (ST5) led to an increase in optimal temperature of the enzymes by 2 and 5°C for the ST4 and ST5,...

Author(s): Chenyan Zhou, Mingcai Zhang, Yongtao Wang, Weiyun Guo, Zhenhua Liu, Yan Wang, and Wu Wang

April 2013

Chemical constituents from the fungus Ganoderma tropicum (Jungh.) Bres. and their cytotoxic activities

 The chemical investigation on the fruiting bodies of white rot fungus Ganoderma tropicum(Jungh.) Bres. led to the isolation of ten natural compounds by column chromatography on silica gel and sephadex LH-20. Their structures were elucidated by nuclear magnetic resonance (NMR) analysis as well as comparison with those reported in the literatures. Compounds 1-10 were isolated for the first time...

Author(s): Qing-yun Ma#, Li-li Hu#, Ling-mei Kong, Sheng-zhuo Huang, Hao-fu Dai, Yan LI and You-xing Zhao

April 2013

Antimicrobial and cytotoxic activities of methanol extract of Alhagi maurorum

Alhagi maurorum Medik (Fabaceae) is the single species of genus Alhagi found in Iraq, locally known as Aqual. Various studies have shown A. maurorum used in folk medicines. However, its potential health benefits have not been studied in details. Methanol extracts ofA. maurorum from the aerial part were screened for total phenolic and flavonoids contents, antioxidant, antimicrobial and...

Author(s): Ghassan M. Sulaiman

April 2013

Production of alkaline protease by Bacillus subtilis using solid state fermentation

 The present study describes the optimization of nutritional and cultural parameters for the production of alkaline protease by Bacillus subtilis under solid state conditions. Among cultural conditions, incubation temperature, incubation period and moisture level of the substrate were optimized and it was found that maximum production of alkaline protease was observed at 37°C and moisture to...

Author(s): Saba Imtiaz, Hamid Mukhtar and Ikram-ul-Haq

April 2013

Virus-like particles detected in postlarvae of abalone (Haliotis diversicolor supertexta Lischke) potentially associated with mass mortalities

  Mass mortalities among cultured postlarvae of abalone, Haliotis diversicolor supertextaLischke of between 10 and 30 days post fertilization, characterized by a white appearance and falling of the diatom films on which they grow, has occurred since 2002 on the south coast of China, including Taiwan. However, its causes remain debated. In this paper, we reported our latest findings on two different types...

Author(s): Jing Zhou#, Renjun Wang# and Junpeng Cai

April 2013

Serosurveillance for some diseases in livestock living within protected areas designated for wildlife reintroduction in Saudi Arabia

The aim of this study is to investigate the prevalence of some diseases in domestic livestock in two protected areas in Saudi Arabia where gazelles have been reintroduced. King Khalid Wildlife Research Centre (KKWRC) was established in 1986 to breed and undertake scientific research on Saudi wildlife for reintroduction purposes. Mountain gazelles (Gazella gazella) were reintroduced to the Ibex Reserve 150 km south...

Author(s): Osama B Mohammed, Abdulaziz N Alagaili, Mohamed A Sandouka, Sawsan A Omer, Maha H Elamin, and Eltyeb ME Abu Elzein

April 2013

Biochemical characterization and identification of bacterial strains isolated from drinking water sources of Kohat, Pakistan

As the pure drinking water is inevitable for good health, it is necessary to evaluate it for bacterial contamination. This study was conducted on the different drinking water sources of Kohat, a Northern-Western District of Pakistan. Sampling was done from different drinking water sources including tap water, tube well water, home-well, bore-well and springs. Physiochemical analyses including pH, temperature,...

Author(s): Tassadaq Hussain, Aneela Roohi, Shehzad Munir, Iftikhar Ahmed, Jafar Khan, Veronique Edel-Hermann, Kil Yong Kim and Muhammad Anees

April 2013

The essential amino acids requirements for Oenococcus Oeni growth and organic acids metabolism

 Lactic acid bacteria are characterized by numerous nutritional requirements. The influence of amino acids on the growth of Oenococcus oeni SD-2a and metabolism of organic acids were determined. The microorganism in basal medium grew to 2.47×108CFU·ml-1 biomass with maximum growth rate of 0.0072 h-1. When glutamic acid and cysteine were independently omitted from basal medium, no growth...

Author(s): Guoqiang Zhang,

April 2013

Detection of high levels of methicillin and multi-drug resistance among clinical isolates of Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) has become a frequent cause of serious infections worldwide. We identified clinical isolates of S. aureus using conventional methods based on morphological and biochemical characteristics. Resistance of isolates to oxacillin was tested by growth on Oxacillin Resistance Screening Agar Base (ORSAB) and disc diffusion method. Oxacillin MICs were...

Author(s): Iman Moneer El Karamany, Yasser Musa Ibrahim, Ahmed Megahed Abouwarda,Tamer Mohammed Essam, and Magdy Ali Amin

April 2013

Antifungal activity of Datura metel L. organic and aqueous extracts on some pathogenic and antagonistic fungi

 The present study was undertaken to assess the in vitro antifungal activity of petroleum ether, chlo-roform, methanol and aqueous extracts of Datura metel L. leaves and flowers, against two Trichoderma species (Trichoderma harzianum and Trichoderma viride) and three formae speciales of Fusarium oxysporum, that is, Fusarium oxysporum f. sp....

Author(s): Asma Rinez, Mejda Daami-Remadi, Afef Ladhari, Faten Omezzine, Imen Rinezand Rabiaa Haouala

April 2013

Growth characteristics of Escherichia coli O157:H7 on sliced meat at temperature abuse conditions

This study investigated the growth and survival of Escherichia coli O157:H7 and aerobic bacteria (AB) in air (AP), vacuum (VP) and modified atmosphere packed (MAP) with sliced meat stored at 4, 6, 8 and 12°C. A slight reduction in the numbers of E. coli O157:H7 was observed after 21 days at 4°C in the VP and MAP sliced meat while significant (p<0.05) growth of bacterium...

Author(s): Osman Erkmen

April 2013

Bacteria found on banks’ automated teller machines (ATMs)

Increasing number of people have used automated teller machines (ATMs) by years, but we know less about their bacterial colonization status. For this purpose, we performed bacterial swab cultures from a total of 100 ATM devices in our city. All devices were found positive for Bacillus spp., and coagulase negative staphylococci (CNS) were isolated from nine devices, including one methicillin resistant...

Author(s): Mehmet Sait TekerekoÄŸlu, Yusuf Yakupogullari, Baris Otlu, Yucel Duman and Nilay Gucluer

April 2013

Seroprevalence of Yersinia pestis in dogs and small rodents in one hyperendemic plague focus of Democratic Republic of Congo

Plague is endemic in Ituri of the Democratic Republic of Congo. In order to evaluate the role of commensal rodents and domestic dogs in the spread of the disease, we studied the seroprevalence at Rethy. Results showed that the 27/28 small rodents were seronegative (using immunoglobulin G anti-F1 enzyme-linked immunosorbent assay), but one of them, Nile rat was positive. These rodents serve as intermediaries,...

Author(s): Bernard Davoust, Georges Diatta, Jean-Christophe Shako, Minoarisa Rajerison, Aaron Aruna Abedi, Stomy Karhemere, Renaud Piarroux and Didier Raoult