March 2013
Inter simple sequence repeat polymorphism in Alternaria genomic DNA exposed to lethal concentrations of isothiocyanates
Isothiocyanates (ITCs) is a group of defense related compounds synthesized byBrassicas that have positive effects on human health which suggest that they are environmentally friendly compounds to control fungi infections. However, the development of ITC-resistant strains of fungi is a matter of concern. The objective of the present work was to study the response...
March 2013
Ultrastructural observations on the resting cyst of the soil ciliate, Territricha stramenticola and the freshwater ciliate, Urostyla grandis
The ultrastructure of non-dividing cells and resting cysts of soil ciliate Territricha stramenticola and freshwater ciliate Urostyla grandis were observed using scanning and transmission electron microscopy. The results show that the resting cell of T. stramenticola shared many similarities with that of U. grandis. Inside the cytoplasm of both resting cysts, there existed...
March 2013
Levels of tumor necrotic factor alpha and interleukin-10 in vaginal discharge of women with infertility disorders and infection
The mucosal immune system of the female reproductive tract is uniquely adapted to facilitate specialized physiological functions whilst eliminating threatening sexually transmitted and environmental pathogens. In the present study, tumor necrotic factor alpha (TNF-α) and interleukin (IL-10) were evaluated in vaginal discharge of infertile women infected with Chlamydia trachomatis and Candida...
March 2013
Phenotypic and genotypic characterization of clinical multidrug resistant Acinetobacter baumannii from Algerian intensive care units
The aim of this study was to evaluate molecular epidemiology and mechanisms of β-lactams resistance in multidrug resistant Acinetobacter baumannii strains causing pneumoniae in two intensive care units (ICUs) of two Algerian University hospitals. Between January 2010 and May 2011, 23 strains were collected. Antibiotics susceptibility testing was performed by the disk...
March 2013
Screening and diversity of plant growth promoting endophytic bacteria from peanut
In order to study potential abilities to promote the growth of the plants and population diversity of endophytic bacteria in peanut plant at four different growth stages, 94 endophytic bacteria strains were isolated. Result indicate that numbers of endophytic bacteria of different growth stages ranged from 2.6 to 7.1×104 cfu/g FW with the maximum presented at the full pod stage. Based on 16S- restriction...
March 2013
Isolation and identification of Mycoplasma agalactiae by polymerase chain reaction (PCR) in suspected sheep samples in Kerman Province, Iran
The infectious agalactia syndrome occurs worldwide but mainly in the Mediterranean regions, north, central and east Africa, and western Asian countries such as Iran.Mycoplasma agalactiae is the chief causal agent of contagious agalactia. The aim of this research was to use the polymerase chain reaction (PCR) method to isolate and identifyM. agalactiae in suspected sheep samples in Iran. They...
March 2013
Characterization and fermentability of sucrose thermolysis oligosaccharides caramel by human intestinal microbiota
Sucrose thermolysis oligosaccharides caramel (STOC) was prepared by thermal treatment (160°C) under vacuum of acidified sucrose using citric acid as caramelization catalyst. Total carbohydrate content was 59.95% in STOC, which was constituted by kestose, mono- and di-oligosaccharide. In addition to carbohydrates, STOC also contained structurally unknown melanoidins. STOC was further subjected to in...
March 2013
Statistical optimization of levansucrase production from Bacillus subtilis ATCC 6633 using response surface methodology
Response surface methodology (RSM) was applied to optimize the conditions of levansucrase production by Bacillus subtilis ATCC 6633, an uninvestigated strain ofBacillus. The effects of the medium sucrose concentrations (100 to 350 g/L), pH (4.0 to 7.0), fermentation time (24 to 48 h) and agitation (100 to 200 rpm) were studied simultaneously using factorial analysis. The results showed that the optimum...
March 2013
Lack of seqA and dam genes induces the formation of cyclic fatty acids in Salmonella typhimurium at the entry of stationary phase
Membrane fatty acid composition of dam and/or seqA mutants of Salmonella typhimuriumwas investigated for exponential and stationary phase cells. Our results show that by reaching the stationary phase, the lack of dam and seqA genes enhanced the production of cyclic fatty acids (CFA), whereas the wild-type cells produced CFAs normally. Data from this study suggest...
March 2013
Analysis of Parvovirus B19 Infection in Renal Transplant Recipients
The clinical manifestation of Parvovirus B19 infection depends on the host's physiologic status and immunologic response. B19 infection can result in serious complications in renal transplant recipients. Participants in this study were 88 kidney recipients who regularly attended the nephrology clinics of a tertiary hospital in eastern Saudi Arabia. Viral-specific IgG and IgM antibodies were measured using a...
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