July 2008
Improvement of the quality and shelf life of concentrated yoghurt (labneh) by the addition of some essential oils
Three essential oils, namely thyme, marjoram and sage, were added to concentrated yoghurt (labneh) at concentrations of 0.2, 0.5 and 1.0 parts per million (ppm). Subsequently, the chemical, microbiological and organoleptic properties of freshly prepared labneh and of the labneh stored at 5ËšC ± 1 for up to 21 days were determined. Addition of essential oils affected the pH, soluble nitrogen-to-total nitrogen,...
July 2008
Chemical analysis and antimicrobial activity of the essential oil of Syzigium aromaticum (clove)
Steam distillation of the dry flower buds of Syzigium aromaticum (clove) yielded 7% (w/w) of the pure light yellow oil. Gas chromatography-mass spectrometry (GC-MS) analysis of the oil revealed that the components were eugenol, caryophyllene, eugenol acetate and alpha-humelene, with eugenol being the main component. The antimicrobial sensitivity of the volatile oil against some Gram-negative bacteria...
July 2008
An improved method for the extraction of nematodes using iodixanol (OptiPrep Ô)
A new nematode extraction technique was established, which is based on an iso-osmotic density-gradient medium (OptiPrepTM). This technique resulted in significantly higher numbers of clean eggs and vermiform nematodes that retain higher viability (48.6%) than samples processed with the sucrose method (28.7%). Nematodes survived exposure to OptiPrepTM for 22 hours without significant mortality whereas all nematodes...
July 2008
Solubilization of inorganic phosphates and plant growth promotion by strains of Pseudomonas fluorescens isolated from acidic soils of Cameroon
A trial of a screening and selection strategy for phosphate-solubilizing bacteria based on phosphate solubilization ability, and the subsequent effect of these strains on plant growth promotion under in situ conditions was conducted. Of all the bacteria tested, three Pseudomonas fluorescens strains (CB501, CD511 and CE509) were selected. On agar plates, two strains (CB501 and CE509) showed an ability...
July 2008
Use of starter cultures of lactic acid bacteria and yeasts as inoculum enrichment for the production of gowé, a sour beverage from Benin
Lactobacillus fermentum, Weissella confusa, Kluyveromyces marxianus andPichia anomala, previously isolated during natural fermentation of traditional gowé, were tested as inoculum enrichment for controlled fermentation of gowé. The final product was subjected to chemical analysis and sensory evaluation. Growth of the lactic acid bacteria (LAB) and yeasts were verified by...
July 2008
Purification and properties of a non-stereospecific dehalogenase enzyme E (DehE) from Methylobacterium sp. HJ1
The bacterial isolate HJ1, which was identified as a Methylobacterium sp., grew on 2, 2-dichloropropionic acid as the sole carbon source and produced a 2-haloalkanoic acid hydrolytic dehalogenase. This non-stereospecific dehalogenase E (DehE) catalysed the hydrolytic dechlorination of 2, 2-dichloropropionic acid and D, L-2-chloropropionic acid to produce pyruvate and lactate, respectively. The enzyme was...
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