Abstract

To determine the comparative wound healing efficacy of ampucare and becaplermin drugs in diabetic rats, diabetes was induced by alloxan 75 mg/kg body weight via intraperitonial route. 500 mm² wound were created on the dorsal area of all animals. Wound lesions were cleaned with normal saline and treated with respective drug for 14 days. Our results revealed that the catalase activity, reduced glutathione, protein, ascorbic acid and nitrate; hydroxyproline and hexosamine levels were significantly increased along with significant decrease in xanthine oxidase activity, and malonaldialdehyde level in the granulation tissue of ampucare treated group as compared with becaplermin treated group on the 14th day. Wound size and bacterial count of ampucare treated group were also significantly reduced as compared to diabetic controlled and becaplermin treated groups. In the histological examination, well organized fibrous tissue proliferation, epithelization and complete scar formation were observed in ampucare treated group in comparison to becaplermin treated group. So these findings concluded that ampucare showed better wound healing efficacy than becaplermin in diabetic rat model.

Key words: Ampucare, becaplermin, wound healing, diabetes, biochemical and antioxidant, oxidative stress.