African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

A novel filarial topoisomerase II inhibitor produced by native isolateMicrococcus luteus B1252

Ramasamy Sivasamy1, Jayaraman Angayarkanni1* and Muthuswamy Palaniswamy2
1Cancer Therapeutics Laboratory, Department of Microbial Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore - 641 046, India. 2Department of Microbiology, School of Life Sciences, Karpagam University, Coimbatore - 641 021, India.
Email: [email protected], [email protected]

  •  Accepted: 12 October 2011
  •  Published: 30 November 2011

Abstract

A novel inhibitor of filarial topoisomerase II was isolated from the culture filtrate of native isolate Micrococcus luteus B1252. The methanolic extract of the cell pellet was partially purified by silica column chromatography and preparative high performance liquid chromatography (HPLC). The active fraction obtained from HPLC was subjected to gas chromatography-mass spectroscopy (GC-MS) analysis which revealed the presence of 11 compounds. The fatty acid ester derivatives (Z) 15-tetracosenoic acid, methyl ester present in the partially purified extract of M. luteus B1252 is suggested to be the active principle which inhibits topo II.

 

Key words: Filariasis, topoisomerase II, Micrococcus luteus B1252, fatty acids, (Z) 15-tetracosenoic acid, methyl ester.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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