African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

DNA fingerprinting for the authentication of Ruta graveolens

Salim Khan1*, Khanda Jabeen Mirza2  and Malik Zainul Abdin2
1Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia. 2Centre for Tansgenic Plant Development, Jamia Hamdard, New Delhi-110062, India.
Email: [email protected]

  •  Accepted: 05 August 2010
  •  Published: 15 August 2011

Abstract

Ruta graveolens is a small aromatic shrub and has been used medicinally and magically, since ancient times. In this study, random amplified polymorphic DNA (RAPD) was employed to develop reproducible markers for authentication of this species from its adulterant Euphorbia dracunculoides. The random decamer oligonucleotide primers (42) were screened for identification of genuine and adulterant samples using the DNA isolated from the dried leaf, seed and stem of both samples. Out of 42 primers, 10 gave faint band, 12 gave species-specific reproducible unique band and the remaining did not amplify the DNA. RAPD couldthus, serve as a complementary tool for quality control.

 

Key words: Adulterant, Euphorbia dracunculoides, herbal drugs, random amplified polymorphic DNA.

Abbreviation

RAPD, Random amplified polymorphic DNA; TCM, traditional Chinese medicine; PCR, polymerase chain reaction; CTAB,cetyltrimethylammonium bromide; EDTA, ethylenediaminetetraacetic acid; PVP,polyvinylpyrrolidone.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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