African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Improvement of electrophoresis performance by spectral analysis

Layal Abo Khayal1, Rene Kizek2 and Ivo Provaznik3*      
1Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Brno, Czech Republic. 2Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University, Brno, Czech Republic. 3International Clinical Research Center – Center of Biomedical Engineering, St. Anne's University Hospital Brno, Brno, Czech Republic.
Email: [email protected]

  •  Accepted: 10 April 2012
  •  Published: 26 June 2012

Abstract

This paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using the real-time spectral analysis of the samples to increase its performance. A laser beam illuminated the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.

 

Key words: Horizontal electrophoresis, laser, spectral analysis, agarose gel.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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