African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Trichoderma spp. from rhizosphere soil and their antagonism against Fusarium sambucinum

  Zhang Ru1,2 and Wang Di2,3*    
  1Vegetable Institute of Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu Province, China. 2Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, Gansu Province, China. 3College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China.  
Email: [email protected] , [email protected]

  •  Accepted: 03 February 2012
  •  Published: 01 March 2012

Abstract

 

One hundred and forty six (146) isolates of Trichoderma spp. were obtained from rhizosphere soils of potato plants in the middle areas of Gansu Province, China. By means of dual culture method, they were examined for antagonism against Fusarium sambucinum, which causes potato dry rot. Ten of the isolates were found to be evidently antagonistic to the pathogen. Based on morphological characteristics and molecular analyses, the antagonistic isolates were identified as Trichodermaharzianum Rifai, Trichoderma longibrachiatum Rifai, Trichoderma atroviride Karstenand Trichoderma virens. Among these isolates, D -3-1 (T. longibrachiatum) showedthe strongest inhibition of the growth of Fusarium sambucinum.

 

Key words: Trichoderma, potato, dry rot, biological control, Fusarium sambucinum