African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Experimental study of the production of biomass by Sacharomyces cerevisiae in a fed batch fermentor

M.O. Daramola1, 2* and L. Zampraka1
  1Bioprocess Engineering Department, Wageningen University and Research Centre, The Netherlands. 2Department of Process Engineering, Stellenbosch University, Private Bag X1, Matieland 7602, Stellenbosch, South Africa.
Email: [email protected]

  •  Accepted: 26 November 2007
  •  Published: 17 April 2008

Abstract

 

The aim of this study is to investigate production of Baker’s yeast using a theoretically programmed fed-batch fermentor to produce as much as possible Baker’s yeast biomass using a minimal amount of substrate with low energy for stirring and aerating. Theoretical data obtained through material balance and kinetics equations were compared with experimental data and possible disagreements discussed with possible physical explanations. The result showed that the experimental biomass yield is greater than the theoretical one. An experimental yield of 92.2% was obtained for growing 191 mol of biomass with 207.8 mol of sugar with low oxygen uptake rate. Also, the obtained specific growth rate (μ) of 0.39 h-1 exceeds by far the maximum specific growth rate of the yeast under aerobic condition as obtained theoretically.

 

Key words: Fermentation, aerobic, fed-batch, yeast, Crabtree.