Enzymatic activity and elicitor of phytoalexins of Lippia sidoides Cham. and endophytic fungi

Talita Pereira de Souza Ferreira; Ronice Alves Veloso; Gil Rodrigues dos Santos; Laiza Priscila dos Santos; Tatiani Pereira de Souza Ferreira; Alessandra Macedo Barros; Richard Dias Possel; Raimundo Wagner de Souza Aguiar

doi:10.5897/AJB2018.16402

African Journal of
Biotechnology

Abbreviation: Afr. J. Biotechnol.
Language: English
ISSN: 1684-5315
DOI: 10.5897/AJB
Start Year: 2002
Published Articles: 12487

Full Length Research Paper

Enzymatic activity and elicitor of phytoalexins of Lippia sidoides Cham. and endophytic fungi

Talita Pereira de Souza Ferreira

Talita Pereira de Souza Ferreira
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Ronice Alves Veloso

Ronice Alves Veloso
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Gil Rodrigues dos Santos

Gil Rodrigues dos Santos
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Laiza Priscila dos Santos

Laiza Priscila dos Santos
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Tatiani Pereira de Souza Ferreira

Tatiani Pereira de Souza Ferreira
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Alessandra Macedo Barros

Alessandra Macedo Barros
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Richard Dias Possel

Richard Dias Possel
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Raimundo Wagner de Souza Aguiar

Raimundo Wagner de Souza Aguiar
Universidade Federal do Tocantins (UFT), Campus UniversitÃ¡rio de Gurupi, 77410-530 Gurupi, TO, Brazil.
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Article Number - 357E4FD56723
Vol.17(15), pp. 521-530 , April 2018
https://doi.org/10.5897/AJB2018.16402

Received: 20 January 2018
Accepted: 03 April 2018
Published: 11 April 2018

Abstract

Secondary metabolites may play a number of roles, among them, is the activation of plant defense mechanisms, such as phytoalexins and the synthesis of antioxidative enzymes. This study aimed to test the eliciting activity of phytoalexins of Lippia sidoides Cham. and six endophytic fungi associated with it and to verify the synthesis of the enzymes, as a response to the antioxidative and antifungal system. The assays were performed using spectrophotometric methods. Of the concentrations of essential oil used, 7.5 mg mL^-1 was 66.48% higher than the Acorda® positive control for the induction of glyceollin. The endophytic fungus, Verticillium sp. showed the best result for 3-deoxythianocyanidine production. Excellent results were demonstrated by the LS-14 fungus for superoxide dismutase enzyme activity (130 U g^-1 E^-1 min^-1); by L. sidoides for the activity of ascorbate peroxidase enzyme (3821 μmol ASA g^-1 E^-1 min^-1); by L. sidoides for the activity of catalase enzyme (2429 μmol H₂O₂ g^-1 E^-1 min^-1); by Fusarium sp. 2 for the activity of phenol peroxidase (87 μmol H₂O₂ g^-1 E^-1 min^-1) and by Fusarium sp. 1 for the activity of chitinase (0.214 U min^-1). Both the essential oil of L. sidoides and the extracts of the endophytic fungi had a positive response to the induction of phytoalexin and presence of enzymes related to antioxidative activity were verified and shown.

Key words: Catalase, superoxide dismutase, peroxidase phenols, ascorbate peroxidase, chitinase.

Abbreviation

SOD, Superoxide dismutase; QUIT, chitinase; APX, ascorbate peroxidase; POX, phenol peroxidase; CAT, catalase; Ver, Verticillium sp.; Col, Colletotrichum sp.; Fus 1, Fusarium sp. 1; Fus 2, Fusarium sp. 2; Lip, Lippia sidoides; LS-6, code of the unidentified fungus; LS-14, code of the unidentified fungus; ROS, reactive oxygen species.