African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

Genetic diversity of Pakistani common myna (Acridotheres tristis) revealed by RAPD-PCR

Ayesha Imtiaz1, Aleem Ahmed Khan1, Muhammad Babar2, Maria Riaz1, Noreen Akhtar1, Muhammad Arshad4 and Imran Khaliq1,3*
1Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan. 2Institute of Biotechnology, Bahauddin Zakariya University, Multan, Pakistan. 3Biology Department, Government Post Graduate College, Dera Ghazi Khan, Pakistan. 4Bilogical Sciences Department, University of Sargodha, Sargodha, Pakistan.
Email: [email protected]

  •  Accepted: 03 June 2011
  •  Published: 01 August 2011

Abstract

Acridotheres tristis is a widely distributed species of Pakistan but no genetic data of this species is available in the literature. In this study, we applied 8 selected RAPD markers from a total of 20 tested markers, to decipher the genetic structure and genetic diversity of different populations. The RAPD markers revealed total polymorphism at P = 87.73%. The overall Nei’s genetic diversity (H) and Shannon’s index (S) levels were high and remained at H = 0.369 and S = 0.540. The genetic distances (D) between populations were also observed to be high. These distances ranged from D = 0.137 between Dera Gahzi Khan and Khanewal to D = 0.273 between Gujranwala and Khanewal. This study will be useful in conservation plans in future under changing environmental scenario.

 

Key words: Acridotheres tristis, genetic diversity, genetic distance, RAPD markers.