African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Biochemical and cytological analysis of five cultivars of Cicer (chickpea)

Jahangir, A. D.
  • Jahangir, A. D.
  • Department of Botany, Institute of Basic Science, Bundelkhand University, Jhansi (U. P.) 284128, India.
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Shahzada Arshid
  • Shahzada Arshid
  • Department of Botany, University of Kashmir, Srinagar 190006, India.
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Kudesia, R.
  • Kudesia, R.
  • Department of Botany, Institute of Basic Science, Bundelkhand University, Jhansi (U. P.) 284128, India.
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Srivastava, M. K.
  • Srivastava, M. K.
  • Department of Botany, Institute of Basic Science, Bundelkhand University, Jhansi (U. P.) 284128, India.
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Aijaz A. Wani
  • Aijaz A. Wani
  • Department of Botany, Institute of Basic Science, Bundelkhand University, Jhansi (U. P.) 284128, India; Department of Botany, University of Kashmir, Srinagar 190006, India.
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  •  Received: 21 June 2013
  •  Accepted: 17 February 2014
  •  Published: 12 March 2014

Abstract

During the present study, protein estimation and protein profiling of five cultivars of Cicer namely Dhawal, ujjwal, Shubhra, DCP-92-3 and IPC-04-20 have been carried out. The protein content on per gram fresh weight basis was found highest in the Dhawal measuring 35.2± 3.83 mg followed by DCP-92- 3 and lowest was found in the IPC-04-20 cultivar. The protein profiling of seed proteins using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed a high polymorphism between the five cultivars. The total number of polypeptide bands recorded was 36, the 4 bands were monomorphic and the rest32 were polymorphic with an average polymorphism of 88.88%. The Jacards similarity ranged from 0.25000 to 0.56000. The similarity index calculated was highest between Ujjwal and IPC-04-20 (37.5%) where as lowest was calculated between Shubhra and IPC-04-20 (22.22%) variety. The unweighted pair group method with arithmetic mean (UPGMA) clustering method revealed two major clusters in the dendrogram that is, cluster 1 and 2, comprising two varieties each. The Shubhra occupies a distinct place as depicted in the dendrogram. Moreover, cytological studies of the five cultivars by calculating the mitotic index were carried out. The mitotic index varied between 4.82 to 10.83% and was found highest in DCP-92-3 while as minimum value was observed in Ujjwal.

 

Key words: SDS-PAGE, UPGMA dendrogram, mitotic index, chickpea.

Abbreviation

Abbreviations: UPGMA, Unweighted pair group method with arithmetic mean; SDS-PAGE; sodium dodecyl sulphate polyacrylamide gel electrophoresis.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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