African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Assessment of genetic diversity among sugarcane cultivars using novel microsatellite markers

Manish Dev Sharma
  • Manish Dev Sharma
  • Molecular Biology and Genetic Engineering College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, Uttrakhand, India; Sam Higginbottom Institute of Agriculture, Technology and Science, Allahabad, India.
  • Search for this author on:
  • Google Scholar
Upma Dobhal
  • Upma Dobhal
  • Molecular Biology and Genetic Engineering College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, Uttrakhand, India.
  • Search for this author on:
  • Google Scholar
Prashant Singh
  • Prashant Singh
  • Molecular Biology and Genetic Engineering College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, Uttrakhand, India.
  • Search for this author on:
  • Google Scholar
Shailender Kumar
  • Shailender Kumar
  • Molecular Biology and Genetic Engineering College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, Uttrakhand, India.
  • Search for this author on:
  • Google Scholar
A. K. Gaur
  • A. K. Gaur
  • Molecular Biology and Genetic Engineering College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, Uttrakhand, India.
  • Search for this author on:
  • Google Scholar
S. P. Singh
  • S. P. Singh
  • College of Agriculture, G. B. Pant University of Agriculture and Technology Pantnagar, Uttarakhand, India.
  • Search for this author on:
  • Google Scholar
A. S. Jeena
  • A. S. Jeena
  • College of Agriculture, G. B. Pant University of Agriculture and Technology Pantnagar, Uttarakhand, India.
  • Search for this author on:
  • Google Scholar
Eapen P Koshy
  • Eapen P Koshy
  • Sam Higginbottom Institute of Agriculture, Technology and Science, Allahabad, India.
  • Search for this author on:
  • Google Scholar
S. Kumar*
  • S. Kumar*
  • Molecular Biology and Genetic Engineering College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology Pantnagar, Uttrakhand, India.
  • Search for this author on:
  • Google Scholar


  •  Received: 11 October 2013
  •  Accepted: 14 March 2014
  •  Published: 26 March 2014

Abstract

Genetic diversity based on the characterization of genetic makeup, using molecular markers is of utmost importance for breeders in crop improvement programme. A total of 26 microsatellite primers were used to determine the genetic diversity among 40 sugarcane genotypes including their parents. The polymerase chain reaction (PCR) products were examined for both size and polymorphism using these primers. Overall alleles are amplified with an average of 2.3 per locus in this study. Of the total 26 simple sequence repeat (SSR) markers, only 10 (38.4%) displayed polymorphism, with polymorphism index contents (PIC) values ranging from 0.15 to 0.67. The observed homozygosity (Ho) and gene diversity (Nei’s) for individual loci varied from 0.0000 to 0.277 and 0.129 to 0.473, respectively. Shannon’s informative index (I) was found to be highest (0.661) in SKM04 while the lowest was 0.252 in SKM01 SSR loci with an average of 0.524. Fixation index was also calculated which was in the range of -0.074 to 1.00. A genetic relationship among cultivars and parental genotype was also analyzed by cluster analysis using unweighted pair group method with arithmetic mean (UPGMA), the average-linkage method, with the similarity matrix as input data. The genetic relationship and genetic diversity among the cultivars depicted from this study can be used to select the parents in sugarcane breeding programme. 

 

Key words: Sugarcane, microsatellite, genetic diversity, SSR.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 13 No. 13
Back to articles
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES