March 2014
Assessment of genetic diversity among sugarcane cultivars using novel microsatellite markers
Genetic diversity based on the characterization of genetic makeup, using molecular markers is of utmost importance for breeders in crop improvement programme. A total of 26 microsatellite primers were used to determine the genetic diversity among 40 sugarcane genotypes including their parents. The polymerase chain reaction (PCR) products were examined for both size and polymorphism using these primers. Overall alleles...
March 2014
Molecular and phenotypic characterization of shigatoxigenic Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) from piglets and infants associated with diarrhoea in Mizoram, India
Limited information is available on shiga toxin producing Escherichia coli (STEC) in pigs and infants from Aizawl, Mizoram and North Eastern region of India. This cross sectional study was conducted on faecal samples from pigs and infants to detect and characterize of STEC and enteropathogenic E. coli (EPEC). Serogrouping, molecular and phenotypic characterizations were done by standard molecular and cytotoxic assays....
March 2014
Construction of intergeneric conjugal transfer for molecular genetic studies of Streptomyces mobaraensis producing transglutaminase
To facilitate molecular studies of Streptomyces mobaraensis producing transglutaminase, an effective transformation method was established via intergeneric conjugal transfer using Escherichia coli ET12567 harboring the ØC31-derived integration vector, pSET152. The highest frequency was attained on ISP4 medium containing 20 mM MgCl2, using 2.5 × 108 E. coli as donor and spore treated with heat treatment at...
March 2014
Effects of auxins on in vitro reserve compounds of Phalaenopsis amabilis (Orchidaceae)
The effects of auxin and the endogenous levels of reserve compounds of Phalaenopsis amabilis (L.) Blume (Orchidaceae) were analyzed in vitro. Rootless plants were inoculated in modified MS media supplemented with IBA or NAA (0.0, 0.2, 1.0 and 5.0 mg L-1) and with 2,4-D (0.000, 0.032, 0.160 and 0.800 mg L-1). The biochemical parameters of endogenous levels of soluble carbohydrates and starch and of total soluble protein...
March 2014
Identification and distribution of Tomato yellow leaf curl virus TYLCV and Tomato yellow leaf curl Sardinia virus TYLCSV infecting vegetable crops in Morocco
Leaf samples of 177 tomato plants were collected during 2006-2007 in tomato yellow leaf curl disease (TYLCD) infected fields, as well as 100 leaf samples of sweet pepper, common bean, zucchini and the wild species Solanum elaeagnifolium, in order to study the population structure and genetic variation of Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato yellow leaf curl virus (TYLCV) in Morocco. Molecular...
March 2014
Gibberellic acid, amino acids (glycine and L-leucine), vitamin B2 and zinc as factors affecting the production pigments by Monascus purpureus in a liquid culture using response surface methodology
The combined effects of zinc, gibberellic acid, vitamin B2, amino acids (glycine and L-leucine) on pigment production were evaluated in a liquid culture of Monascus purpureus. In this study, response surface design was used to optimize each parameter. The data were analyzed using Minitab 14 software. Five parameters were applied by using response surface methodology for pigment production in liquid cultures. Four...
March 2014
Antibacterial activity of some selected plants traditionally used as medicine in Manipur
Methanolic leaf extracts of the plant species Elsholtzia blanda Benth., Elsholtzia communis (Collett & Hemsl) Diels., Polygonum posumbu Buchanam-Hamilton ex D. Don and Zanthoxylum acanthopodium DC. using methanol as a solvent were tested against 10 human pathogenic bacteria for potential antibacterial activity. The study revealed that all extracts show varied degree of antibacterial activity against the tested...
March 2014
Comparison of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) for diagnosis of Fusarium solani in human immunodeficiency virus (HIV) positive patients
Fusarium solani is the most virulent Fusarium sp., frequently reported in the fatal disseminated fusariosis in immunocompromised patients. However, it has been always considered, as a very rare or case report infection among HIV positive patients. Anyhow, because of its irreparable consequences, early diagnosis is very important. In this study, loop-mediated isothermal amplification (LAMP) method was developed for rapid...
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