African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Antimicrobial activities of grape (Vitis vinifera L.) pomace polyphenols as a source of naturally occurring bioactive components

Faisal Kabir*
  • Faisal Kabir*
  • Department of General Animal Science and Animal Nutrition, Patuakhali Science and Technology University, Babugonj, Barisal-8210, Bangladesh.
  • Google Scholar
Mosammad Shahin Sultana
  • Mosammad Shahin Sultana
  • Department of Agricultural Economics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
  • Google Scholar
Heri Kurnianta
  • Heri Kurnianta
  • Department of Bioscience and Food Production Science, Shinshu University, 8304 Minamiminowamura, Ina, Nagano 399-4598, Japan.
  • Google Scholar


  •  Received: 02 April 2015
  •  Accepted: 28 June 2015
  •  Published: 30 July 2015

Abstract

Grape pomace is a potential source of winery by-products having useful bioactive components. Antimicrobial activities of enzyme-assisted grape pomace polyphenols (GPP) were assessed against Escherichia coli IFO 3301 and Staphylococcus aureus IFO 12732 using plate count and spectrophotometry assays. GPP have shown credential growth inhibition against E. coli and S. aureus, respectively. The higher growth inhibition was mediated by the higher GPP concentrations against both E. coli and S. aureus, which implies dose dependency. GPP also exhibited bactericidal effects against both the Gram-positive and Gram-negative bacteria, whereas, Gram-positive bacteria have shown more susceptibility than Gram-negative bacteria. It is revealed that GPP is a potential source of natural antimicrobial agents.
 
Key words: Grape pomace, polyphenols, antimicrobial activity.
 

Abbreviation

LB, Luria broth; GPP, grape pomace polyphenols; DMSO, dimethyl sulfoxide; PBS, phosphate buffer saline.