African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12344

Full Length Research Paper

Effect of VIUSID-Agro™ on the conversion of somatic embryos of coffee (Coffea arabica L.) cv. Red Caturra rojo-884

Laisyn Posada-Pérez
  • Laisyn Posada-Pérez
  • Instituto de Biotecnología de Las Plantas, Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní KM 5½, Santa Clara, Villa Clara, Cuba.
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Raúl Barbón Rodríguez
  • Raúl Barbón Rodríguez
  • Instituto de Biotecnología de Las Plantas, Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní KM 5½, Santa Clara, Villa Clara, Cuba.
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Alina Capote Pérez
  • Alina Capote Pérez
  • Instituto de Biotecnología de Las Plantas, Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní KM 5½, Santa Clara, Villa Clara, Cuba.
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Anabel Pérez Pérez
  • Anabel Pérez Pérez
  • Instituto de Biotecnología de Las Plantas, Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní KM 5½, Santa Clara, Villa Clara, Cuba.
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Yenny Padrón Montesino
  • Yenny Padrón Montesino
  • Instituto de Biotecnología de Las Plantas, Universidad Central “Marta Abreu” de Las Villas. Carretera a Camajuaní KM 5½, Santa Clara, Villa Clara, Cuba.
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Bulent Kukurtcu
  • Bulent Kukurtcu
  • Catalysis, S. L. Macarena, 14. 28016, Madrid, España, Spain.
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Dion D. Daniels
  • Dion D. Daniels
  • Faculty of Science and Technology, University of Belize, Hummingbird Avenue, Belmopan, Belize C.A., Belize.
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Guillermo Reyes
  • Guillermo Reyes
  • Facultad de Agronomía, Universidad Nacional Agraria (UNA), Managua, Nicaragua.
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Rafael Gómez-Kosky
  • Rafael Gómez-Kosky
  • Estación Territorial de Investigaciones de La Caña de Azúcar (ETICA Centro Villa Clara), Instituto de Investigaciones de La Caña de Azúcar (INICA), Autopista Nacional KM 246. Ranchuelo, Villa Clara, Cuba.
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  •  Received: 03 February 2021
  •  Accepted: 21 May 2021
  •  Published: 30 June 2021

Abstract

A critical factor in the in vitro propagation protocol via somatic embryogenesis of coffee is the survival and growth of plants under ex vitro conditions. The objective of this work was to determine the effect of VIUSID Agro™ during the conversion of somatic embryos of coffee cv. Red Caturra rojo-884 in semi-controlled culture conditions. Two solutions (0.5 and 0.8 mL L-1) of the biostimulant were evaluated on in vitro plant. Applications were done twice daily for the first three days and thereafter once a day from day 7 to 90 days after transplanting. Survival rate at 15 days and morpho-physiological characteristics at 30, 60 and 90 days after transplanting were evaluated. All treatments with VIUSID-Agro™ and the control resulted in 100% survival. The concentration of 0.5 mL L-1 of VIUSID-Agro™ registered the better results in morpho-physiological variables. These results constitute the first report of the use of VIUSID Agro™ on in vitro plant of coffee during acclimatization phase.

 

Key words: Ex vitro acclimatization, coffee, conversion, somatic embryos, VIUSID Agro™.