African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Bacteria response to non-thermal physical factors: A study on Staphylococcus aureus

  Focea Ramona1, Poiata Antoniea2, Motrescu Iuliana1, Nastuta Andrei1, Creanga Dorina1* and Popa Gheorghe1        
  1University Alexandru Ioan Cuza Iasi, Faculty of Physics, Biophysics and Medical Physics Laboratory, Iasi, Romania. 2University “Gr. T. Popa” Iasi, Faculty of Pharmacy, Iasi, Romania.
Email: [email protected], [email protected]

  •  Accepted: 16 February 2012
  •  Published: 01 March 2012

Abstract

 

The response of Staphylococcus aureus germ to the action of ionizing radiation and cold plasma jet was studied in the frame of a comparative experimental research. The inactivation effect of electromagnetic ionizing radiations provided by medical X-ray device has resulted in up to 75% diminution of bacterial cell density, while for the same radiation doses provided by 6 MeV electron beam delivered by hospital radiotherapy accelerator, three times slighter effect has resulted. It was further shown that bacteria inactivation could be obtained by applying cold plasma treatment for shortened irradiation times; 100 s instead of 50 min having similar effect of about 73% efficiency on S. aureus, as resulted from counting the survival colony forming units. The S. aureus antimicrobial susceptibility was found slightly changed for X-rays but not for electron beam irradiation or cold plasma exposure.

 

Key words: X-ray, electron beam, plasma jet, resistance to antibiotics.