Abstract

Papaver orientale is an important medicinal plant and source of the valuable alkaloids. In this study, callus production and regeneration of P. orientale under in vitro conditions was investigated with different levels of kinetin (Kin), 6-benzylamine purine (BA) and α-naphthalene acetic acid (NAA) on Murashige and Skoog (MS) and Gamborg's B5 (B5) media. For callogenesis, explants as cotyledons-hypocotylswere placed on the solidified MS and B5 media with hormones under 16-h photoperiod and 20°C conditions. Results showed that the best media for callus induction of P. orientale consisted of the MS basic media supplemented with 0.5 mg/l BA and 0.5 or 1 mg/l NAA. Shoots were regenerated in cultures grown on MS medium containing 0.5 mg/l Kin and (0.5 or 1.0 mg/l) NAA and were induced to root on B5 medium containing 1 mg/l Kin and 1.0 mg/l NAA.

Key word: Callus induction, in vitro regeneration, Papaveraceae, Papaver orientale.

Abbreviation

BA, 6-Benzylamine purine; 2,4-D, 2,4-dichlorophenoxyacetic acid;DMR, Duncan’s multiple range; MS, Murashige and Skoog; B5, Gamborg's B5 medium; Kin, kinetin; NAA, α-naphthalene acetic acid