African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Comparative study on semen characteristics of Kolbroek and Large White boars following computer aided sperm analysis® (CASA)

Matshidiso Bailekae Masenya1,2, M. L. Mphaphathi1,3, M. H. Mapeka1,3, P. H. Munyai1,4, M. B. Makhafola1,2, F. V. Ramukhithi1,3, P. P. Malusi1, D. O. Umesiobi2 and T. L. Nedambale1,3,4*
1Agricultural Research Council, Animal Production Institute, Germplasm Conservation & Reproductive Biotechnologies, Private Bag X2, Irene, 0062, South Africa. 2Department of Agriculture, Central University of Technology, Private Bag X20539, Bloemfontein, 9300, South Africa. 3Tshwane University of Technology, Department of Animal Sciences, Private Bag X680, Pretoria 0001, South Africa. 4University of the Free State, Department of Animal, Wildlife and Grassland Sciences, Private Bag X 339, Bloemfontein, South Africa.
Email: [email protected]

  •  Accepted: 09 September 2011
  •  Published: 26 October 2011

Abstract

Consistent estimates of boar fertility potential from objective semen evaluation could be a valuable tool for boar selection. The objective of this study was to evaluate semen characteristics of Kolbroek and Large White boars following computer aided sperm analysis® (CASA). Eight ejaculates were collected separately from individual Kolbroek (n = 4) and Large White (n = 4) boars using the gloved-hand technique. Following semen collection, semen was evaluated for macroscopic and microscopic characteristics. Analysis of variance (ANOVA) was used to test the differences between the breeds (P<0.05). The bodyweight of Kolbroek (154.7 ± 8.5) was significantly lower compared to Large White (189.9 ± 7.7) boar. There was also a positive correlation between bodyweight and semen volume of both Kolbroek (r = 0.2197) and Large White (r = 0.2577) boar. However, no significant differences were observed in Kolbroek and Large White boar semen volume (140 and 170 ml), sperm concentration (0.727 and 0.761 × 109 sperm cell/ml), pH (7.0 and 7.0), total motility (95 and 91%) and morphology (84 and 82%). In conclusion, the bodyweight of Kolbroek and Large White boar was positively correlated with ejaculated semen volume. Sperm characteristics of both Kolbroek and Large White boar were similar. Sperm class analyser® provided a precise and more objective information of sperm motility characteristics.

 

Key words: Sperm, Large White, Kolbroek, motility rate, boar.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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