African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Inheritance of DNA methylation in DH and its backcrossed lines of Brassica napus

Zhen Guo1, Liping Zeng1, Maoteng Li2, Yan Long1* and Jinling Meng1
1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China. 2College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Email: [email protected]

  •  Accepted: 08 April 2010
  •  Published: 01 August 2011

Abstract

Nineteen (19) pairs of methylation sensitive amplified polymorphism (MSAP) primers were used for analysing DNA methylation in two DH lines and their two back-crossed generations, namely BC1 and BC2. Constitutive DNA methylation was stable across three generations and inherited changes in it were of six types. 60 fragments were chosen for sequencing and then, were analysed using the Blast database and the strength of their expression was assessed. Two types of genes, those related to retro-transposons and those involved in other functions, were identified but the degree of expression of those genes was lower in the progeny than in the parents.

 

Key words: DNA methylation, methylation sensitive amplified polymorphism (MSAP), backcross line, Brassica napus.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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