African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Statistical optimization of activity and stability of β-xylanase produced by newly isolated Thermomyces lanuginosus THKU-49 using central composite design

Khwanchai Khucharoenphaisan1, Shinji Tokuyama2 and Vichien Kitpreechavanich1*
1Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand. 2Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529 Japan.
Email: [email protected]

  •  Accepted: 16 September 2008
  •  Published: 20 October 2008

Abstract

A factorial design was performed to find the best conditions of pH and temperature for β-xylanase activity and to maintain its activity for prolonged periods of time of pure xylanase produced by newly isolated Thermomyces lanuginosus THKU-49. The central composite design (CCD) used for the analysis of treatment combinations showed that a regression models of optimization of xylanase activity and xylanase stability were good agreement to experimental results with R2 = 0.98 and 0.99, respectively. The maximum activity of xylanase was obtained at 66oC and pH 6.3.  The temperature and pH for maximum enzyme stability was 70oC and pH 7.3, respectively. Under this condition xylanase having half-life of 825 min indicatedthe highest thermostable xylanase.

 

Key words: β-xylanase, optimization, central composite design.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 7 No. 20
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES