African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro investigation of the repellent effects of the essential oil of Lippia javanica on adults of Hyalomma marginatum rufipes

S. R. Magano1*, F. Nchu2 and J. N. Eloff3
1Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Florida Campus, Johannesburg, South Africa. 2Faculty of Applied Sciences, Cape Peninsula University of Technology, Cape Town, South Africa. 3Programme for Phytomedicine, Department of Paraclinical Sciences, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa.
Email: [email protected]

  •  Accepted: 13 June 2011
  •  Published: 15 August 2011

Abstract

A suitable in vitro tick climbing repellency bioassay was developed to evaluate the repellent effects of the essential oil (n-hexane extracts of distillate) of Lippia javanica(Burm. F.) Spreng (Verbernaceae) on adults of Hyalomma marginatum rufipesKoch, 1844. The GC-MS analysis of the distillate of the essential oil of Lippia javanica revealed that myrcene (13.4%), 1,8-cineole (8%), dyhydrotagetone (7.9%), ipsenone (9.6%), bicyclo (3.1.1) heptanes-2-one (20.8%) and 2-butanone (13.3%) were the major constituents. A significant (P < 0.05) dose dependent repellency response was observed for the essential oil of L. javanica on adults of H. marginatum rufipes when data were subjected to Kruskal Wallis analysis.

 

Key words: Hyalomma marginatum rufipes, Lippia javanica, repellency bioassay, essential oil, GC-MS.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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