African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Identification of sugarcane interspecies hybrids with RAPDs

H. Y. Zhang1,2, F. S. Li1,3*, l. L. He3, H. Q. Zhong3, Q. H. Yang3 and S. C. He3
  1Key Laboratory of Agricultural Biodiversity for Pest management, Ministry of Education, Yunnan Agricultural University, Kunming, Yunnan Province, People’s Republic of China, 650201. 2Biotechnology laboratory; Southwest Forestry College; White Dragon Temple; Kunming, Yunnan Province-650224; People’s Republic of China. 3College of Agronomy and Biotechnology of Yunnan Agricultural University, Kunming, Yunnan Province, People’s Republic of China, 650201.
Email: [email protected].

  •  Published: 17 April 2008

Abstract

 

Identification of “Saccharum officinarum × Erianthus fulvus” F1 hybrids was performed with random amplified polymorphic DNA (RAPD) analysis. Of 280 RAPD primers used, two primers, OPA-19 and OPN-11, were found to be the most suitable for identification of the hybrids. And the hybrids facticity-check-out rate was 70.6 and 68.3%, respectively.

 

Key words: Sugarcane hybrids, identification, RAPD.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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