African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12500

Full Length Research Paper

Theoretical evaluations of electro-manipulation from Jurkat T cells exposed to pulsed electric fields (PEFs) with different time durations

  Minghe Wu1*, Hongchun Yang1, Baohua Teng1, Yu Lei1, Xiaoming Zheng2, Mei Zhang3, Yang Li1 and Chengli Ruan1      
  1School of Physical Electronics, University of Electronics Science and Technology of China (UESTC), 610054, Chengdu, China. 2School of Dentistry and Health Science, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia. 3Faculty of Computer, Guangdong University of Technology, Guangzhou 510006, China.
Email: [email protected]

  •  Accepted: 03 February 2012
  •  Published: 01 March 2012

Abstract

 

The multilayer dielectric sphere model (MDSM) was used to analyze responses of Jurkat T cells to pulsed electric fields (PEFs). The dependence of voltages across nuclear plasma, nuclear envelope, cytoplasm and plasma membrane on different time durations of PEFs was calculated by employing the inverse laplace transformation (ILT) in time domain with Maple software. Cytochrome C release was considered as a secondary effect of PEFs when mitochondria substructures were taken into account. It is proposed that two-step process of Cytochrome C release can be realized by bioelectric impulse effect on mitochondria.

 

Keywords: Laplace transformation, Jurkat T, pulsed electric fields (PEFS), cytochrome C. 

 

Abbreviation

MDSM, Multilayer dielectric sphere model; PEFs, pulsed electric fields; ILT, inverse laplace transformation.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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