Abstract

 

This research paper mainly focused on developing a media by optimizing parameters like sweet potato concentration, sodium nitrate concentration, pH, temperature for the maximum production of amylase by Aspergillus oryzae MTCC 1847. Optimization of the medium components such as sweet potato (carbon source), sodium nitrate (nitrogen source) and parameters like pH and temperature were studied for the amylase production by using response surface methodology (RSM) based central composite design (CCD). By using the surface plots and response optimizer of MINITAB 14 Software, the maximum enzyme activity of 0.893 IU/ml was predicted when sweet potato concentration was 2.01%, sodium nitrate 0.6%, pH 7.2 and temperature 25°C.

 

Key words: Sweet potato, Aspergillus oryzae, response surface methodology, central composite design.

Abbreviation

 

 ANOVA, Analysis of variance; CCD, central composite design; RSM,response surface methodology; DNS, dinitrosalicylic acid.

 

List of symbols: F, Fisher’s function; P, corresponding level of significance; T,student’s test; X₁, sweet potato; X₂, sodium nitrate; X₃, pH; X₄, temperature. Y,predicted response; β, coefficient.