African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Investigation of parietal polysaccharides from Retama raetam roots

BOKHARI-TAIEB BRAHIMI Hassiba*
  • BOKHARI-TAIEB BRAHIMI Hassiba*
  • Laboratoire des Productions, Valorisations Vegetales et Microbiennes (LP2VM), Departement de Biotechnologies Vegetales, Universite des Sciences et de la Technologie d?Oran Mohamed Boudiaf, B.P. 1505, El-Mn?aour, Oran 31000, Algerie.
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FAUGERON Celine
  • FAUGERON Celine
  • Laboratoire de Chimie des Substances Naturelles, Universite de Limoges, 123, avenue Albert Thomas, 87060 Limoges cedex, France.
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HACHEM Kadda
  • HACHEM Kadda
  • Laboratoire des Productions, Valorisations Vegetales et Microbiennes (LP2VM), Departement de biologie, faculte des sciences, universite Dr.TAHAR MOULAY de Saida, BP 138, cite Ennasr, 20000 Saida, Algerie.
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KAID-HARCHE Meriem
  • KAID-HARCHE Meriem
  • Laboratoire des Productions, Valorisations Vegetales et Microbiennes (LP2VM), Departement de Biotechnologies Vegetales, Universite des Sciences et de la Technologie d?Oran Mohamed Boudiaf, B.P. 1505, El-Mn?aour, Oran 31000, Algerie.
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GLOAGUEN Vincent
  • GLOAGUEN Vincent
  • Laboratoire de Chimie des Substances Naturelles, Universite de Limoges, 123, avenue Albert Thomas, 87060 Limoges cedex, France.
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  •  Received: 24 May 2015
  •  Accepted: 15 July 2015
  •  Published: 22 July 2015

Abstract

This study characterizes the cell wall hemicellulose and pectins polymers of Retama raetam. This species develops a particularly important root system and is adapted to arid areas. The cellulose, hemicelluloses and pectins were extracted. The cellulose remains the major component of the wall (27% for young roots and 80% for  adult roots), hemicelluloses (14.3% for young roots and 3.6%  for adult roots) and pectins (17.3% for young roots and 4.1% for adult roots). The monosaccharidic composition of water soluble extracts determined by gas liquid chromatography (GLC) and completed by infrared (FTIR) spectroscopy of hemicellulosic shows the presence of xylose as a major monosaccharide in the non-cellulose polysaccharides (47.8% for young roots and 59.5% for adult roots). These results indicate the presence of the homogalacturonans and rhamnogalacturonans in pectin. This study constitutes the preliminary data obtained in the biochemical analysis of the parietal compounds of the roots of a species which grows in an arid area in comparison with those of its aerial parts.

 

Key words: Retama  raetam,  roots, cell wall, investigation,  polysaccharides, monosaccharidic.