July 2015
Rhodanese is a possible enzyme marker for cyanide environmental stress on aquatic life
Rhodanese is a cyanide detoxifying enzyme. The role of man through his anthropogenic activities in and around water bodies have increased in recent times. These have led to constant exposure of water body to cyanide and cyanide compounds with increase to loss of many aquatic lives. There are limited methods employed in quick detection of cyanide in water. The aim of this paper was to present rhodanese, an enzyme, as a...
July 2015
Authenticating the origin of different shrimp products on the Tunisian markets by PCR/RFLP method
This study describes a polymerase chain reaction using restriction fragment length polymorphism (PCR-RFLP) assay based on the 16S rRNA mitochondrial gene to identify commercial food products of wide range of Penaeidae and Pandalidae shrimp species commercialised in the Tunisian market. Phylogenetic analyses on 16S rRNA mitochondrial gene were used to study the relationships among the considered species. Penaeidae shrimp...
July 2015
Diversity analysis of the immunoglobulin M heavy chain gene in Nile tilapia, Oreochromis niloticus (Linnaeus)
A full-length cDNA encoding the immunoglobulin (IgM) heavy chain gene of Nile tilapia was successfully cloned using the 5’ and 3’ RACE techniques. The complete cDNA of the Nile tilapia IgM heavy chain gene is 1,921 bp in length and has an open reading frame (ORF) of 1,740 bp, which corresponds to 580 amino acid residues. The deduced amino acid sequence of the Nile tilapia IgM heavy chain includes a typical...
July 2015
A valid measure to eliminate the influence of polysaccharides and polyphenols in recalcitrant longan (Dimocarpus longan L.) during DNA isolation
Large amounts of polysaccharides, polyphenols, tannins, proteins, and other secondary metabolites in recalcitrant longan leaves make it difficult to obtain high quality genomic DNA during extraction. To obtain good quality of nucleic acids from local longan leaves and for its downstream applications, a new protocol was developed. It consists of rapid isolation of stable nuclei, which hinders covalent interactions with...
July 2015
Hydroponic technology for lily flowers and bulbs production using rainwater and some common nutrient solutions
This experiment was carried out to investigate the potential of nutrient film technique (NFT) hydroponic system for flowers and bulbs production of the Asiatic hybrid lily cv. "Blackout" using rainwater and some common nutrient solutions (Hoagland No. 2 Basal Salt Mixture, Murashige and Skoog Basal Salt Mixture and White’s Basal Salt) with rock wool cubes as medium with or without removal of flower buds...
July 2015
Chemical and quantitive study of hepatotoxins from fresh water cyanobacteria on vertebrate animals
Toxicity of the freeze-dried micro-aquatic planktonic cyanobacterium Oscillatoria agardhii, dominating and isolated from Makkah –KSA was studied. Microcystins were detected from freeze-dried cells using high pressure liquid chromatography HPLC. The histopathological examination of mice liver injected on week basis with diluted 1/5 of the lethal dose, (105 mg/ Kg body weight), of toxin extract revealed sever...
July 2015
Biochemical and kinetic characterization of geranylgeraniol 18-hydroxylase (CYP97C27) from Croton stellatopilosus Ohba
Geranylgeraniol 18-hydroxylase (EC 1.14.13.110) that exists solely in Croton stellatopilosus Ohba catalyses the last committed step of plaunotol biosynthetic pathways by conversion of geranylgeraniol (GGOH) to plaunotol. This enzyme and its gene are an attractive target for development of plaunotol production and its detailed biochemical properties need to be understood. Recently, even though the gene...
July 2015
Investigation of parietal polysaccharides from Retama raetam roots
This study characterizes the cell wall hemicellulose and pectins polymers of Retama raetam. This species develops a particularly important root system and is adapted to arid areas. The cellulose, hemicelluloses and pectins were extracted. The cellulose remains the major component of the wall (27% for young roots and 80% for adult roots), hemicelluloses (14.3% for young roots and 3.6% for adult roots) and...
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